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For stable and transient transfections in a wide variety of cell lines
The ESCORT series of reagents were designed to meet the specific requirements needed for effective transfections in a broad spectrum of cell lines. Each one was formulated to optimize transfection efficiency based on the cell host and experimental parameters. These parameters include: stable vs. transient, serum vs. no serum, and mammalian vs. insect. All of the ESCORTs offer low toxicity levels and the flexibility to move from one common cell type to another.
ESCORT™ Reagent Selection Table
High Efficiency Transfection of Insect Cells with ESCORT™ Reagents
Transfection of Sf9 insect cells with ESCORT and ESCORT IV
Sf9 cells were seeded at a density of 3 x 106 in 60 mm dishes. After one hour, the attached cells were transfected in serum-free medium with 3 µg of plasmid DNA containing a constitutively expressed LacZ gene. The DNA:lipid ratio was 1:4 (w/v). Following a five-hour incubation, the medium was replaced with serum containing medium. The cells were stained at 40 hours post-transfection for the presence of β-galactosidase.
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ESCORT™ (Product No. E9770)
- Efficiently transfects wide variety of cell types
- Low toxicity
- Suitable for stable and transient transfection
- Use in serum-containing or serum-free media
- Economical
- Ideal for Sf insect cells
5 µl is sufficient to transfect in a 35 mm culture dish; 15 µl is sufficient in a 60 mm dish.
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ESCORT™ II (Product No. L6037)
- High efficiencies
- Transfects a wide spectrum of cells
- Works well in serum
Sufficient for 150 transfections (2 µg of DNA/dish) 1.5 ml sufficient for 300 µg DNA transfection
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ESCORT™ III (Product No. L3037)
- Recommended for primary cell lines
- Reagent of choice for PC-12
- Low toxicity
Do not freeze.
1 ml is sufficient for 250 - 1000 transfections.
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ESCORT™ IV (Product No. L3287)
- Reagent of choice for insect cells
- Recommended for cultured cell lines
- Low toxicity
Do not freeze.
1 ml is sufficient for 160 - 250 transfections.
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| ESCORT™ Transfection Reagent Selection Table |
| Cell Line |
ESCORT™ |
ESCORT™ II |
ESCORT™ III |
ESCORT™ IV |
| BHK |
|
√ |
|
|
| CHO |
|
√ |
|
√ |
| COS-7 |
√ |
√ |
|
|
| F9 |
|
√ |
|
|
| HEK293T |
|
√ |
|
|
| HeLa |
|
√ |
|
|
| L929 |
|
√ |
|
|
| NIH3T3 |
√ |
√ |
|
√ |
| PC-12 |
|
|
√ |
|
| Sf9 |
√ |
|
|
√ |
| T.ni. |
√ |
|
|
√ |
| Vero |
|
√ |
|
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| Note: These are reagents recommended for the cell line indicated. If a reagent is not checked for a particular cell line, the reagent may not have been tested in that particular cell line. Please refer to catalog listings for additional cell lines the reagents are known to transfect. Transfection efficiency may vary depending on the plasmid and cell line used. |
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