|
Sigma-Aldrich offers baculovirus transfer vectors for expression of FLAG® or MAT™ (Metal Affinity Tag) fusion proteins at high levels in insect cells using the baculovirus expression vector system. These vectors combined with top-performing ESCORT™ transfection reagents provide superior advantages for insect cell expression.
- FLAG® tag for high-level detection and purification using ANTI-FLAG® antibodies, resins, and 96-well plates
- MAT tag for powerful one-step purification using HIS-Select™ Nickel and Cobalt affinity gels
- N-terminal and C-terminal configurations
- Compatible with commercially available ORF 1629 deleted baculoviral DNA
- Enterokinase removal of N-terminal FLAG tags
|
|
View a Vector Selection Guide.
Baculovirus Transfer Vectors
pPolh transfer vectors are for the construction of recombinant baculovirus used in insect cell expression systems. The strong polyhedrin (polh) promoter results in high-level expression of target genes during the very late phase of infection. The AcNPV ORF 603 and ORF1629 regions flank the polh promoter and MCS for homologous recombination with baculoviral DNA and generation of recombinant baculovirus. pPolh vectors also contain the pUC origin of replication and b-lactamase gene ampr for propagation and selection in bacterial cells. The following baculovirus transfer vectors are offered as N- or C-terminal FLAG or MAT tag versions for convenient downstream detection and purification of recombinant protein.
| Product |
MCS Region |
T6824, pPolh-FLAG™-1
N-terminal Met-FLAG |
|
E6155, pPolh-FLAG™-2
C-terminal FLAG |
|
T6699, pPolh-MAT™-1
N-terminal MAT |
|
T6574, pPolh-MAT™-2
C-terminal MAT |
|
MAT Tag
The MAT tag or Metal Affinity Tag (HNHRHKH) has been created for purification of recombinant MAT fusion proteins using HIS-Select Nickel and Cobalt Affinity Gels. HIS-Select products allow for highly selective purification of histidine-tagged fusion proteins such as MAT fusions.
Enterokinase Removal of FLAG Tag
The recognition sequence for enterokinase, Asp-Asp-Asp-Asp-Lys, is found at the C-terminal end of the FLAG epitope tag. Removal of FLAG is possible in all fusion proteins containing an N-terminal FLAG sequence.
back to top
back to Expression Vectors
|
