Bacterial FLAG® Expression Kits

• E. coli Amino-Terminal FLAG Expression Kit
• E. coli Carboxy-Terminal FLAG Expression Kit
• E. coli FLAG-Shift™ Expression Kit

The E. coli Amino-Terminal FLAG Expression Kit provides the specialized FLAG components needed to perform expression of a recombinant N-terminal FLAG fusion in Escherichia coli. The pFLAG-ATS™ (E 5769) and pFLAG-MAC™ (E 5644) Expression Vectors provide a choice of secreted or cytoplasmic expression of N-terminal FLAG fusion proteins, respectively, under control of the i>tac promoter. Also provided in the kit are controls for expression, detection, affinity purification and enterokinase removal of N-terminal FLAG.

Components ANTI-FLAG® M1 monoclonal antibody ANTI-FLAG® M2 affinity gel ANTI-FLAG® M2 monoclonal antibody Enterokinase FLAG® peptide pFLAG-ATS–BAP™ control plasmid pFLAG–ATS™ expression vector (OmpA leader) pFLAG–MAC™ expression vector (no leader) C-24 sequencing primer N-26 sequencing primer N-terminal FLAG–BAP™ control protein

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The E. coli Carboxy-Terminal FLAG Expression Kit provides the specialized FLAG components needed to perform expression of a recombinant C-terminal FLAG fusion in Escherichia coli. The pFLAG-CTS™ (E 5269) and pFLAG-CTC™ (E 5394) Expression Vectors provide a choice of secreted or cytoplasmic expression of C-terminal FLAG fusion proteins, respectively, under control of the tac promoter. Also provided in the kit are controls for expression, detection, and affinity purification.

Components ANTI-FLAG® M1 monoclonal antibody FLAG® peptide pFLAG-CTC™ expression vector (no leader) pFLAG–CTS-BAP™ control plasmid pFLAG–CTS™ expression vector (OmpA leader) N-26 sequencing primer C-24 sequencing primers C-terminal FLAG–BAP™ control protein

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The E. coli FLAG-Shift™ Expression Kit provides the specialized FLAG components needed for expression of an open reading frame (ORF) in all three reading frames as N-terminal FLAG fusion proteins in Escherichia coli. The pFLAG-Shift₁₂™ (E 8023) and pFLAG-Shift_12c™ (E 7898) Expression Vectors provide a choice of secreted or cytoplasmic expression of N-terminal FLAG fusion proteins, respectively, under control of the tac promoter. Both vectors contain 12 thymidine residues just upstream from the MCS region that result in frame-shifting during translation. Therefore, simultaneous expression of all three reading frames occurs. This feature is useful when the ORF is unknown as in genomic, cDNA or shotgun libraries. Also provided in the kit are controls for expression, detection, affinity purification and enterokinase removal of N-terminal FLAG.

Components ANTI-FLAG® M1 monoclonal antibody ANTI-FLAG® M2 affinity gel ANTI-FLAG® M2 monoclonal antibody Enterokinase FLAG® peptide FLAG-Shift™12–BAP control plasmid FLAG-Shift™12 expression vector (no leader) FLAG-Shift™12 expression vector (OmpA leader) C-24 sequencing primer N-26 sequencing primer N-terminal FLAG–BAP™ control protein

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