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The new MAT tag or Metal Affinity Tag (HNHRHKH) provides for highly sensitive detection using the Anti-MAT™ antibody and robust one-step purification of recombinant MAT fusion proteins using HIS-Select™ Nickel and Cobalt Affinity Gels.
Bacterial Expression-tac Promoter
- Cytoplasmic expression
- N- or C- terminal fusion proteins
- Ampicillin resistance, T1T2 transcriptional terminator, pMB1(derivative of pBR322) origin of replication, and lacI gene for repression of the tac promoter
- Expression levels using the tac promoter may be in excess of 10 mg/L of culture when using IPTG as a de-repressor
- Expression in any established Escherichia coli expression host
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| Product |
MCS Region |
E5530, pTAC-MAT-1
N-terminal MAT |
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E5405, pTAC-MAT-2
C-terminal MAT |
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Bacterial Expression-T7 Promoter
- Cytoplasmic expression
- N- or C- terminal fusions, dual tag configurations
- FLAG® vectors for highly sensitive detection and purification using ANTI-FLAG® antibodies, resins, and plates
- Ampicillin resistance, T1T2 transcriptional terminator, pMB1 (derivative of pBR322) origin of replication, and lacI gene for repression of the T7 promoter
- Higher yields of recombinant protein than tac system
- lac operator (lacO) sequences reduce leaky expression
- Requires hosts with a source of the T7 polymerase such as (DE3) lysogenic strains
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| Product |
MCS Region |
E5780,
pT7-MAT™-1
N-terminal MAT |
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E5655,
pT7-MAT™-2
C-terminal MAT |
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E5280,
pT7-FLAG-MAT™-1
N-terminal Met-FLAG, C-terminal MAT (dual tag) |
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E4905,
pT7-MAT™-FLAG-2
N-terminal MAT,C-terminal FLAG (dual tag) |
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E5155,
pT7-MAT-FLAG™-1
N-terminal MAT-FLAG (dual tag) |
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E5030,
pT7-FLAG-MAT™-2
C-terminal FLAG-MAT (dual tag) |
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Mammalian Expression
- Transient, cytoplasmic expression
- N- or C- terminal dual tag configurations
- FLAG® vectors for highly sensitive detection and purification using ANTI-FLAG® antibodies, resins, and plates
- Strong human cytomegalovirus (CMV) promoter regulatory region drives constitutive protein expression levels as high as 1 mg/L in COS cells (in less potent cell lines, protein levels are typically ~0.1 mg/L)
- pMB1 (derivative of pBR322) origin for replication in bacterial cells
- Ampicillin resistance selection in bacteria
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| Product |
MCS Region |
C5864, pCMV-FLAG-MAT™-1
N-terminal Met-FLAG, C-terminal MAT (Dual tagged) | |
C5989, pCMV-MAT-FLAG™-1
N-terminal MAT-FLAG (Dual tagged) | |
C6114, pCMV-FLAG-MAT™-2
C-terminal FLAG-MAT (Dual tagged) | |
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Insect Cell Expression
- N-terminal and C-terminal configurations
- Compatible with commercially available ORF 1629 deleted baculoviral DNA
- Strong polyhedrin (polh) promoter results in high-level expression of target genes during the very late phase of infection
- AcNPV ORF 603 and ORF1629 regions flank the polh promoter and MCS for homologous recombination with baculoviral DNA and generation of recombinant baculovirus
- pUC origin of replication and β-lactamase gene (ampr for propagation and selection in bacterial cells
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| Product |
MCS Region |
T6699, pPolh-MAT™-1
N-terminal MAT |
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T6574, pPolh-MAT™-1
C-terminal MAT |
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