Mammalian FLAG® Expression Kits

• Mammalian Amino-terminal FLAG® Transient Expression Kit
• Mammalian Amino-terminal FLAG® Stable Expression Kit
• Mammalian Carboxy-terminal FLAG® Transient Expression Kit

The Mammalian Amino-Terminal FLAG® Transient Expression Kit provides all of the specialized FLAG components needed to perform expression of a recombinant N-terminal FLAG fusion in mammalian cells. The pFLAG-CMV™-1 (E 7273) and pFLAG-CMV™-2 (E 7398) Expression Vectors allow transient expression of secreted or cytoplasmically expressed fusion proteins. Also provided in the kit are controls for expression, detection, and affinity purification.

Components ANTI-FLAG® M2 monoclonal antibody ANTI-FLAG® M5 monoclonal antibody C-CMV-24 sequencing primer N-CMV-30 sequencing primer FLAG® peptide Met-FLAG-BAP™ control protein pFLAG-CMV™-1-BAP control plasmid pFLAG-CMV™-2-BAP control plasmid pFLAG-CMV™-2 expression vector (no leader) pFLAG-CMV™-1 expression vector (preprotrypsin leader)

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The Mammalian Amino-Terminal FLAG Stable Expression Kit provides the specialized FLAG components needed for expression of a recombinant N-terminal FLAG fusion in mammalian cells. The pFLAG-CMV™-3 (E 8770) and pFLAG-CMV™-4 (E 1775) Expression Vectors allow transient or stable expression of secreted or cytoplasmically expressed fusion proteins. Stable expression is accomplished by neomycin selection (G 418). Also provided in the kit are controls for expression, detection, and affinity purification.

Components ANTI-FLAG® M2 monoclonal antibody ANTI-FLAG® M5 monoclonal antibody C-CMV-24 sequencing primer N-CMV-30 sequencing primer FLAG® peptide Met-FLAG-BAP™ control protein pFLAG-CMV™-3-BAP control plasmid pFLAG-CMV™-4-BAP control plasmid pFLAG-CMV™-4 expression vector (no leader) pFLAG-CMV™-3 expression vector (preprotrypsin leader)

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The Mammalian Carboxy-Terminal FLAG® Transient Expression Kit provides the specialized FLAG components needed for transient expression of a recombinant C-terminal FLAG fusion in mammalian cells. The pFLAG-CMV™-5a,b,c Expression Vectors (E 3762) make all reading frames of the MCS available at each restriction site for cloning of an open reading frame. All three vectors lack a translational start signal, so the start signal and any secretion leaders must be inserted along with the gene of interest. Also provided in the kit are controls for expression, detection, and affinity purification.

Components ANTI-FLAG® M2 affinity gel ANTI-FLAG® M2 monoclonal antibody C-CMV-24 sequencing primer N-CMV-30 sequencing primer FLAG® peptide pFLAG-CMV™-5b–BAP control plasmid pFLAG-CMV™-5a expression vector pFLAG-CMV™-5b expression vector pFLAG-CMV™-5c expression vector C-terminal FLAG–BAP™ control protein

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