Extract-N-Amp™ Seed PCR Kit – Extract genomic DNA from seeds and amplify targets of interest by PCR.

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Extract-N-Amp Seed™ PCR Kits
	DNA and RNA Purification

Description: The Extract-N-Amp Seed™ PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from seeds and amplify targets of interest by PCR (Figure 1).

Novel – single-step extraction of seed genomic DNA to PCR
Fast – seed to PCR in 15 minutes
Convenient – no organic extraction, column purification or precipitation of DNA

The Extract-N-Amp Seed PCR Kit offers a novel extraction system that eliminates the need for DNA purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The product includes a specially formulated hot start PCR ReadyMix™ for amplification directly from the extract. Extract-N-Amp products are Sigma Advanced Technology certified.

Procedure: Genomic DNA is extracted from ground seed material by incubation in a mixture of Extraction Solution and Seed Preparation Solution at 55 °C for 10 minutes. After the extraction is stopped by incubation at 95 °C for 3 minutes, an equal volume of Neutralization Solution B is added and the extract is ready for PCR. An aliquot of the extract is then added directly to the optimized PCR mix supplied.

Application: Perfect for genotyping.

Beneficial Features

Starting Material	1 seed
Speed	Extraction of seed genomic DNA for PCR in less than 15 minutes
Versatile	Extract and amplify target DNA sequences from a variety of plant species (Figure 1)
High Specificity	Hot Start antibody for highly specific PCR amplification of genomic DNA
Safe	No phenol/chloroform or other hazardous materials

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Pick the formulation that is right for you.

The Extract-N-Amp Tissue PCR Kits include a specially formulated hot start PCR ReadyMix for amplification directly from the extract.

The PCR ReadyMix comes in two formulations:

REDExtract-N-Amp PCR Ready Mix – contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reactions onto agarose gels for analysis.
Extract-N-Amp PCR Ready Mix – no dye contained.

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Order Information

Extract-N-Amp Seed PCR Kits

Product Code	Name	Number of Extractions	Number of Amplifications	Technical Manual .pdf
XNAS2	Extract-N-Amp Seed PCR Kit	100	100	X

REDExtract-N-Amp Seed PCR Kits

Product Code	Name	Number of Extractions	Number of Amplifications	Technical Manual .pdf
XNASS	REDExtract-N-Amp Seed PCR Kit (contains REDTaq)	10	10	X
XNAS	REDExtract-N-Amp Seed PCR Kit (contains REDTaq)	100	100	X

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Kit Contents

Extract-N-Amp Kit

Seed Preparation Solution
Extraction Solution
Neutralization Solution B
Extract-N-Amp PCR Ready Mix

REDExtract-N-Amp Kit

Seed Preparation Solution
Extraction Solution
Neutralization Solution B
REDExtract-N-Amp PCR Ready Mix

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Sample Data

Multiplex PCR analysis of Extract-N-Amp Seed PCR Kit extracts from various seed types.

Figure 1. Genomic DNA was extracted from the seeds using the protocol as described in the Extract-N-Amp Seed Technical Bulletin. All extracts were then amplified using the specially formulated JumpStart™ PCR mix and PCR primers multiplexed for both a universal chloroplast gene (~400-700 bp) and the acetylcoenzyme A carboxylase gene specific to wheat (964 bp).
Extract-N-Amp Seed PCR Kit

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Stability of Soybean Extracts at 4 °C.

Figure 2. Soybean seeds were extracted according to the procedure in the Technical Bulletin for the Extract-N-Amp Seed PCR Kit. 4 µL aliquots were analyzed immediately by quantitative PCR with SYBR Green detection on an ABI Prism® 7700. DNA standards for quantitative PCR were purified DNA prepared from soybean seeds using the GenElute Plant Genomic DNA Kit (Product code G2N70) and stored as single use aliquots at -20 °C. The soybean seed extracts were stored at 4 °C (recommended storage). Quantitative PCR was repeated after 2.5 and 5 weeks and then 2 and 6 months for the 4 °C samples. These results show that the extracts will be stable for at least 6 months at the recommended storage temperature of 4 °C.

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REDTaq allows for convenient loading onto agarose gels.

Figure 3. Samples are loaded directly on an agarose gel after PCR amplification with RedTaq DNA Polymerase, obviating the need or addition of loading buffers and/or tracking dyes. The RedTaq dye approximately co-migrates with a 125 bp duplex.

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