Extract-N-Amp™ Blood PCR Kit

Figure 1. Extract-N-Amp Blood PCR Kit used to isolate genomic DNA from fresh, 7 day old & frozen blood. DNA was extracted and neutralized from 10 µl of whole blood in 5 minutes at room temperature using the REDExtract-N-Amp Blood PCR kit. The PCR products were then generated using the specially formulated Hot Start PCR mix included in the kit. PCR products generated are 1.8 kb for carnitine palmitoyltransferase II, 1.3 kb for a mitochondrial DNA control region, 547 bp for human surfactant protein B and 320 bp for the 5' untranslated region of human major histocompatibility complex class II.

Figure 2. Blood was drawn from 2 human volunteers into vacutainer tubes containing EDTA. Extractions were performed in duplicate providing 4 samples total. Samples were stored at 37 °C and removed at various time intervals for testing. Stability was determined by monitoring yield from quanitative PCR using an ABI 7700 instrument. The DNA standards used for the quanitative PCR were generated from the same blood drawn as the test samples. The DNA for the standards was purified using the GenElute Blood Genomic DNA Kit (NA2000). The PCR products were generated using primers for a 547 bp product for human surfactant protein B (SPB; Lin & Floros, 2000, BioTechniques, 29:460-466). The results clearly show no loss of amplification of the SPB PCR product even after storage at 37 °C for 6 months.