Description: The Extract-N-Amp™ Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by PCR (Figure 1).
- Novel – single-step extraction of genomic DNA to PCR
- Fast – tissue to PCR in 15 minutes
- Convenient – no long enzymatic digestions
The Extract-N-Amp Tissue PCR Kit offers a novel extraction system that eliminates the need for either long enzymatic digestions or homogenization. The product includes a specially formulated hot start PCR ReadyMix™ for amplification directly from the extract. Extract-N-Amp products are Sigma Advanced Technology certified.
Procedure: Genomic DNA is extracted from a tissue sample that has been incubated in Tissue Preparation Solution and Extraction Solution for 10 minutes at room termperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. An aliquot of the DNA extract is then added directly to the optimized PCR mix supplied.
Application: Perfect for genotyping.
Watch the video protocol: Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit
Beneficial Features
|
Starting Material |
Mouse tail (0.5 to 1.0 cm)
tissue (2 to 10 mg)
buccal (10 µL) |
| Speed |
Extraction of genomic DNA for PCR in 15 minutes |
| Convenient |
No long enzymatic digestions |
| Safe |
No phenol/chloroform or other hazardous materials |
| Specific |
Hot Start antibody for highly specific PCR amplification of genomic DNA |
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