Left Panel: 1 µg of 100 bp DNA ladder was electrophoresed on a 2.0% agarose gel. 100, 200, 300, 400, 500, and 600 bp fragments were excised from the gel, and purified using GenElute Agarose Spin Columns as above. Samples were loaded and analyzed on a 2.0% agarose gel.
Right Panel: Lambda Hind III markers were pre-heated at 65°C and electrophoresed on a 2.0% agarose gel. The 2.0, 4.4, 6.6, 9.4, and 23 kb fragments were excised and purified using GenElute Agarose Spin Columns. Samples were loaded and analyzed on a 2.0% agarose gel.
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