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Description: The GenElute™ Blood Genomic DNA Kit provides a simple and convenient technique to isolate high-quality genomic DNA from fresh or aged (older than 24 hours) whole blood. This kit combines the advantages of a silica-based system with a microspin format, eliminating the need for expensive resins and hazardous organic compounds.
- Fast – less than 40 minutes
- Easy – spin column format
- Cost effective – more preps for the money
Procedure: Whole blood is incubated with the appropriate enzymes to ensure efficient cell lysis and DNA realease from the cells. The whole blood is then lysed in a chatotropic salt-containing solution. DNA is bound to the silica based membrane and the remaining lysate is removed by centrifugation. After washing, to remove contaminants that are associated with aged whole blood samples, the DNA is eluted with buffer into a collection tube.
Application: Purified DNA (Figure 1) is ready for downstream applications such as restriction endonuclease digestions (Figure 2), PCR (Figure 3), Southern Blots and Automated Sequencing reactions.
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