GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit

Description: The GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit offers a novel and efficient method of isolating endotoxin-free plasmid DNA in 40 minutes. GenElute HP Endotoxin-Free Plasmid Kits include a filter syringe for clearing lysates and binding columns that are used with a vacuum format.

• Sophisticated – newest technology ensures improved performance
• Novel – includes patent-pending endotoxin removal system
• Plentiful – up to 1.2 mgs of high-quality, endotoxin-free (≤0.1EU/µg) plasmid DNA in 40 minutes
• Convenient – vacuum format and no alcohol precipitation

Procedure: An overnight E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure. The cleared lysate is mixed with Binding Solution, which includes reagents that inhibit endotoxins from adsorbing to the Binding Column while promoting the adsorption of plasmid DNA. No separate endotoxin removal step is required. After washing, the DNA is eluted in buffer or water.

Application: High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells (Figure 1).

150 ml

Up to 1.2 mg of high-copy plasmid DNA

From harvested bacterial culture to pure plasmid DNA in 40 minutes

Convenient vacuum format does not use gravity flow columns

Lower cost plus higher yields per prep equals more than double the savings