The GenElute™ HP Plasmid Midiprep and Maxiprep Kits offer purification of high quality plasmid DNA in less than 30 minutes (Figure 1). GenElute™ HP Plasmid Kits feature a filter syringe for lysate clearing, and binding columns that can be used with a vacuum or spin purification format.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure. The lysate is cleared with a filter syringe and DNA is bound to the silica-based membrane. The remaining contaminates are removed by a wash step. Finally, the bound DNA is eluted in buffer or water. High quality DNA is suitable for the most demanding applications including transfection (Figure 3), restriction digestion, ligation, automated sequencing.

Features and Benefits

• From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
• Up to 350 µg (Midi) and 1.2 mg (Maxi) yield of high-copy plasmid DNA (Figure 2)
• Offers the flexibility of a vacuum or spin format