GenElute™ PCR Clean-Up

Description: The GenElute™ PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reactions, such as excess primers, nucleotides, DNA polymerase, oil and salts (Figure 1).

Procedure: DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 µl or 10 µg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp) are removed.

Application: Purified DNA can be used in enzymatic reactions, conventional or Automated Sequencing (Figure 2), and Cloning and Microarray Analysis.

100 µL or 10 µg of DNA

Recovers up to 95% of PCR fragments between 100 bp to 10 kb.

Purify up to 100 µL or 10 µg of PCR amplified DNA in 5 minutes.

DNA concentrated in 50 µL of buffer

40% more purifications than market leader