GenElute™ Plant Genomic DNA Miniprep Kit

Description: With the GenElute Plant Genomic DNA Miniprep Kit, high quality genomic DNA can be purified from a variety of plant species (Table 1, Figure 1, and Figure 2). The kit contains all the reagents, columns and tubes necessary to isolate genomic DNA from up to 100 mg of fresh or 20 mg of freeze-dried plant tissue.

Quick look: G2N10, G2N70, G2N350

Procedure: Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. Purified DNA is ready for downstream applications such as PCR (Figure 2), restriction endonuclease digestions, cloning and southern blots.

Features and Benefits:

• Less than 40 minutes from tissue to purified genomic DNA, including disruption in liquid nitrogen
• Typical DNA yields of up to 20 µg per prep
• No RNase treatment required
• 40% more purifications per kit than the leading supplier

Storage: Room Temperature
R: 20/21/22-32-36/38 S: 26-36

Application: Purified DNA (Figure 2) is ready for downstream applications such as PCR (Figure 3), restriction endonuclease digestions, cloning and southern blots.

Figure 1. Purified genomic DNA (0.4 µg/lane) was analyzed on a 0.8% agarose gel. Markers are Lambda EcoR I (Product No. D9281) Hind III digest.

Figure 2. Genomic DNA from soybean leaves was purified using the GenElute™ Plant Genomic DNA Miniprep Kit. A 5 µl aliquot of eluate was used as template in a 20 µl total PCR reaction for 30 cycles. A 5 µl aliquote of each PCR reaction was resolved on a 2% precast agarose gel (Product No. P5722). The PCR marker (M) used (Product No. P9577) ranged from 50 bp to 2 kb.