GenElute Plasmid Miniprep Kit

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GenElute™ Plasmid Miniprep Kit
	DNA and RNA Purification

Description: GenElute™ Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating up to 20 µg plasmid DNA from E. coli cultures.

Fast – 24 preps in 30 minutes
Convenient – spin column format
Cost effective – more preps for the money

Procedure: Bacterial cells are harvested via centrifugation, subjected to a modified alkaline-SDS lysis procedure and the DNA adsorbed onto silica in the presence of high salts. Contaminants are then removed by a simple wash step. Bound DNA is eluted in water or Tris-EDTA buffer.

Application: Plasmid DNA is ready for immediate use in restriction enzyme digestion (Figure 1) cloning, PCR, transcription, conventional and automated sequencing (Figure 2).

Beneficial Features

Culture Volume	1 - 5 ml overnight bacterial culture
Yield	Up to 20 µg of plasmid DNA
Speed	30 minutes for 24 preps
Versatile	Contains additional wash buffer for use with EndA+ E. coli bacterial strains (e.g., HB101, JM101, BL21)
Specific	No detectable genomic DNA or RNA contamination
Safe	No phenol/chloroform required

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Order Information

Product Code	Culture Volume (ml)	Plasmid Yield (µg)	Time Required	Number of Preps	Technical Manual .pdf	Short protocols .pdf
PLN10 PLN70 PLN350	1 to 5	30 to 20	Hands On: < 10 min. Total: < 30 min.	10 70 350	X X X	X X X
PLD35 PLD140	5 to 40	25 to 200	Hands On: < 10 min. Total: < 40 min.	35 140	X X	X X
PLX15 PLX50	25 to 130	200 to 1,200	Hands On: < 10 min. Total: < 40 min.	15 50	X X	X X

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Sample Data

Plasmid DNA purified with GenElute Plasmid Miniprep Kit is suitable for restriction enzyme digestions.

Figure 1. Restriction digestions of pUC19 purified from JM109 using the GenElute Plasmid Miniprep Kit. Lane 1 was an undigested plasmid, lanes 2-4 were cut with Bam HI, Eco RI and Pst I respectively. Lane M was 1 kb ladder (11 fragments from 0.5 to 10 kb). Electrophoresis was on a 1% agarose gel.

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High-quality plasmid DNA for Automated Sequencing.

Figure 2. Electropherogram revealing >600 bases of sequence from pUC-TMV using GenElute Plasmid Miniprep Kit. Cycle Sequencing was performed using 500 ng of template, a T7 sequencing primer, and ABI Big Dye^TM terminator chemistry. Sequencing reactions were resolved on an ABI Prism 377 XL instrument with a 48 cm gel cassette containing 4.5% AutoPAGE Plus acrylamide at 2.4 kV, 48°C for 7 hours.

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