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We provide kits for the isolation of high quality plasmid and Bacterial Artificial Chromosome (BAC) DNA. Suitable for work with large and small scale plasmid, endotoxin-free plasmid, and BAC DNA. The technology uses advanced silica bind and elute with vacuum or spin format. Used for transfections sequencing, restriction digestions, cloning, and gene therapy.
Click on the product name for more information or read testimonials
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Yield |
Speed |
Product |
Starting Material |
Product Description |
Technology |
Applications |
| Large-scale plasmid DNA |
Maxiprep
up to 1.2 mg
Midiprep
up to 350µg |
30
minutes |
GenElute™ HP Plasmid Kit |
150 ml (Maxi)
50 ml (Midi) |
Fast and simple approach for isolating high-quality plasmid DNA |
Advanced silica bind & elute with vacuum or spin format. |
Transfections (robust cells), sequencing, restriction digestion, cloning, and PCR |
| Large-scale endotoxin-free plasmid DNA |
Maxiprep
up to 1.2 mg
Megaprep up to 5 mg
Gigaprep
up to 15 mg |
40 minutes (Maxi)
90 minutes (Mega)
Less than 2 hours (Giga) |
GenElute™ HP Endotoxin -Free Plasmid Maxiprep Kit
GenElute™ HP Endotoxin -Free Plasmid Megaprep Kit
GenElute™ HP Select Plasmid Gigaprep Kit |
150 ml(Maxi) Bacterial Culture
200 mL - 1L (Mega) Bacterial Culture
600 mL-2.5L (Giga) Bacterial Culture |
Isolation of high-quality plasmid DNA
(≤0.1EU/µg) |
Advanced silica bind & elute with vacuum format (Maxi).
Advanced silica bind & elute with vacuum format (Mega).
Advanced silica bind & elute with vacuum format (Giga). |
Transfections (endotoxin-sensitive cells), sequencing, restriction digestion, cloning, PCR, and gene therapy research |
| Small-scale plasmid DNA |
Miniprep (HP)
up to
25 µg
Five-Minute
up to 5 µg |
< 30 minutes (Miniprep)
5 minutes |
GenElute™ HP Plasmid Miniprep Kit
GenElute™ Plasmid Miniprep Kit
GenElute™ Five-Minute Plasmid Miniprep Kit |
1-5 ml Bacterial Culture (Mini)
400 µl (Five-Minute) |
Isolation of plasmid DNA |
Advanced silica bind & elute with vacuum or spin format
Advanced patent-pending process. |
Transfection (GenElute™ HP Miniprep only), sequencing, screening clones, restriction digestion and PCR |
| BAC DNA |
Up to
100 µg |
100-210 minutes |
PhasePrep™ BAC DNA Kit |
5-500 ml Bacterial Culture |
Isolation of Bacterial Artificial Chromosomes |
Silica bind process in a spin column format |
Sequencing, PCR, restriction digestion and cloning |
GenElute™ Five-minute Plasmid Miniprep Kit
"I never used a faster plasmid purification kit before. The Sigma GenElute™ HP Plasmid Purification Kits are really time-saving and easy to handle."
Andreas Knoedler
Oregon Health Sciences University
"I spend less time purifying DNA and I get higher yields with the Sigma GenElute™ HP Plasmid Purification Kits. The kits give me time back that I can spend on other experiments."
Rene Rijnbrand
The University of Texas Medical Branch
Galveston
"Purifying DNA is fairly routine in our labs, but nevertheless very important. Everything comes down to the DNA and we need high volumes and high quality necessary for mouse injections. Sigma’s GenElute™ HP Plasmid Purification Kit provides both in about an hour, saving us all valuable time."
Carol Williams
University of Texas
Southwestern Medical Center
"I get great sequencing reads with the DNA purified from Sigma’s GenElute™ Plasmid Purification Kits."
Michael Byrne
Partners Health Care
Massachusetts General Hospital
"I switched to Sigma’s GenElute™ HP Plasmid Purification Kit because I receive a higher purity and the protocol is very simple."
Tsui-Fen Chou
University of Minnesota
"I switched to Sigma’s GenElute™ HP Plasmid Purification Kits because the yield is much higher, the purity is better or equal to my previous method and, importantly, it takes me only a quarter of the time."
Waldemar Popik
Johns Hopkins University
"I am very glad I tried the Sigma GenElute™ Plasmid DNA Purification Kit. The preps are quick, easy and yield large amounts of high-quality DNA that can be used for all of my applications including transfections, sequencing and cloning."
Peter Baumeister
USC, Norris Cancer Center
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DNA & RNA Purification page
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