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 mRNA Quick Reference Guide

DNA and RNA Purification
 
Click on the product for more information
 
Product Yield Speed Starting Material Product Description Technology Applications
SpyLine™ Poly A+ Capture Kit Captured mRNA for direct RT-PCR on plate (optional elution if desired) 40-90 minutes 1000-50000 cells per well Isolation of mRNA from cultured mammalian cells for direct RT-PCR Capture of poly A+ mRNA onto 96-well PCR plate coated with oligo dT; optimized for high-throughput isolation RT-PCR (in plate), northern blots and microarray analysis (after optional elution)
GenElute™ Direct mRNA Miniprep Kit Up to 5 µg 60 minutes Up to 107 mammalian cells or
50 mg of tissue per prep
Isolation of mRNA from mammalian cells and tissues Hybrid-capture onto oligo dT-polystyrene beads; wash & elute in a spin column format RT-PCR, northern blots and microarray analysis
GenElute™ mRNA Miniprep Kit 1-5% of starting total RNA 40 minutes 5-500 µg total RNA Isolation of mRNA from total RNA Hybrid-capture onto oligo dT-polystyrene beads; wash & elute in a spin column format RT-PCR, northern blots and microarray analysis