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Selected Protocol from "Molecular Cloning: A Laboratory Manual", Third Edition, Joseph Sambrook, David W. Russell.
Removal of Ethidium Bromide from DNA by Extraction with Organic Solvents
Joseph Sambrook
Peter Maccallum Cancer Institute and The University of Melbourne, Australia
David W. Russell
University of Texas Southwestern Medical Center, Dallas
ABSTRACT
Ethidium bromide is usually removed from DNA purified through a CsCl gradient by repeated extraction with organic solvents. The CsCl is subsequently removed by dialysis or by precipitation. This method is highly effective in removing ethidium bromide from DNA purified by equilibrium centrifugation in CsCl-ethidium bromide gradients. |
