Bionic™ Buffer, 10x Electrophoresis Buffer

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Bionic™ Buffer, 10x Electrophoresis Buffer
	Nucleic Acid Electrophoresis

Better...Faster...Stronger!

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.

Resolve bands in minutes
Run gels 2-3x faster than in TBE/TAE
Sharper bands in less time
Compatible with pre-cast gels
Voltage less limited by heat generation

For best results, prepare and run your agarose gel with Bionic Buffer. Alternatively, Bionic Buffer is compatible with standard pre-cast gels or freshly made TBE and TAE gels.

Note: A voltage of 150 V is recommended for small electrophoresis chambers while a mid to large apparatus may be run at 200-250 V. It is recommended that the user test their chamber to determine optimum voltage.

Bionic Buffer FAQ

Order Information

Product Code	Product Name	Size
B6185	Bionic Buffer, 10x Buffer	100 mL 1 L 4 L 10 L

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Sample Data

High Resolution Band Separation in Less Time!

Figure 1. Lanes 1, 4 & 7: 50 - 3,000 bp marker; Lanes 2 & 5: 100 - 1,000 bp marker; Lanes 3 & 6: 50 - 2,000 bp marker. PCR markers were loaded on 0.8% agarose gels prepared with (1x) Bionic Buffer or (1x) TBE Buffer. Bionic Buffer ran at its optimal voltage of 250 volts; TBE was run at 100 volts. In only 10 minutes, Bionic Buffer allows for better and sharper band resolution than traditional TBE.

Works great with pre-cast gels!

Figure 2. This is a 1% TBE pre-cast gel (P5472) electrophoresed with 1x Bionic Buffer for 10 minutes at 250 volts. There is excellent band resolution and separation in a very short time with the pre-cast gel. This gel was loaded and then run with no equilibration time. Gel was loaded as follows: Lane 1: 100-1000 bp DNA ladder (D3687); Lane 2: 50-2000 bp PCR Marker (P9577); Lanes 3,4: 500-10,000 bp 1 KB ladder (D3937).

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