Dyes, Loading Buffers, and Nucleic Acid Gel Stains
Dyes and Loading Buffers
| Product Name |
Technical Bulletin |
Product # |
| Gel Loading Solution |
 |
G2526 |
| Gel Loading Solution Type I |
|
G7654 |
| RNA Sample Loading Buffer Without Ethidium bromide |
|
R1386 |
| RNA Sample Loading Buffer With Ethidium bromide |
|
R4268 |
| Bromphenol Blue sodium salt |
|
B5525 |
| Bromphenol Blue-Xylene Cyanole Dye solution |
|
B3269 |
| Xylene Cyanole FF |
|
X4126 |
| |
Number of Base Pairs |
| Acrylamide Concentration |
Bromophenol Blue |
Xylene Cyanole |
| 3.5% |
100 |
460 |
| 5% |
65 |
260 |
| 12% |
45 |
70 |
| 15% |
20 |
60 |
| 20% |
15 |
45 |
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Nucleic Acid Gel Stains
A wide range of nucleic acid stains for polyacrylamide and agarose are available including BlueView™, Ethidium bromide, SYBR® Green I and II, methylene blue, DAPI, acridine orange and others. The table helps identify the stains, which can be used for various nucleic acid applications, and their detection limits by gel electrophoresis.
| Application |
BlueView™ |
Ethidium bromide |
Methylene blue |
SYBR® Green I |
SYBR® Green II |
Pyronin Y |
DAPI |
Acridine Orange |
| DNA |
+ |
+ |
+ |
+ |
|
|
+ |
+ |
| RNA |
|
+ |
+ |
|
+ |
+ |
|
+ |
| Fluorescent |
|
+ |
|
+ |
+ |
+ |
+ |
+ |
| Colormetric |
+ |
|
+ |
|
|
+ |
|
|
| Agarose |
+ |
+ |
+ |
+ |
+ |
|
+ |
+ |
| PAGE |
|
+ |
+ |
+ |
+ |
+ |
|
+ |
| Detection limits |
0.2 µg DNA |
1 ng DNA |
5 ng DNA |
60 pg DNA |
0.5 ng RNA |
|
0.5 µg DNA |
50 ng DNA |
| Product Name |
Technical Bulletin |
Product # |
| Acridine orange hemi(zinc chloride) salt |
|
A6014 |
| BlueView™ Stain in 10x TAE Buffer Powder Blend |
|
T8935 |
| BlueView™ Stain in 10x TBE Buffer Powder Blend |
|
T9060 |
| Ethidium bromide powder, for molecular biology |
|
E7637 |
| Ethidium bromide tablet (100mg), for molecular biology |
|
E2515 |
| Ethidium bromide tablet (10mg), for molecular biology |
|
E4391 |
| Ethidium bromide aqueous solution, for molecular biology |
|
E1510 |
| InstaStain™ (7cm x 7cm) |
|
I8029 |
| InstaStain™ (14cm x 14cm) |
|
I8154 |
| Extractor for Ethidium bromide decontamination |
|
Z36,156-9 |
| Pyronin Y |
|
P9172 |
| SYBR® Green I nucleic acid gel stain |
|
S9430 |
| SYBR® Green II RNA gel stain |
|
S9305 |
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