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Sigma’s versatile GenomePlex technology has emerged as the preferred method of amplification of DNA from Chromatin Immunoprecipitation (ChIP). A number of recent publications have demonstrated that GenomePlex outperforms the traditional method of amplification, Ligation-Mediated PCR (LM-PCR). The unique ability of GenomePlex to amplify fragmented DNA while maintaining representation makes it perfectly suited for this challenging application.
References
Comparison of sample preparation methods for ChIP-chip assays
O'Geen, H.; Nicolet, C. M.; Blahnik, K,; Green, R.; Farnham, P. J. BioTechniques 2006 Nov, 41(5), 577–80.
(PubMed abstract)
A novel role for the Aurora B kinase in epigenetic marking of silent chromatin in differentiated postmitotic cells
Sabbattini, P; et al. EMBO J. 2007 Nov 14, 26(22), 4657–69.
(PubMed abstract)
Genome-scale ChIP-chip analysis using 10,000 human cells
Acevedo, L. G.; et al. BioTechniques 2007 Dec, 43(6), 791–7.
(No PubMed abstract available)
Protocols
Whole Genome Amplification Protocol for ChIP-chip
This protocol is provided courtesy of Dr. Peggy Farnham,
Associate Director of the UC Davis Genome Center
Professor of Pharmacology, UC Davis School of Medicine
The lab of Dr. Peggy Farnham
UC Davis Genome Center Expression Analysis Core Facility
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