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The GenomePlex Spotlight gathers the latest technical information on whole genome amplification.
Posters
qPCR Quality Assessment of Whole Genome Amplified FFPE DNA Samples and their Use on a BAC aCGH Platform (752 Kb PDF)
Array CGH Analysis of Challenging Samples
Products
The GenomePlex Tissue Whole Genome Amplification Kit (WGA5) contains all of the reagents necessary to amplify genomic DNA directly from formalin-fixed, paraffin-embedded (FFPE), frozen, RNAlater®-preserved or fresh tissue.
Protocols
Whole Genome Amplification Protocol for ChIP-chip
This protocol is provided courtesy of Dr. Peggy Farnham,
Associate Director of the UC Davis Genome Center
Professor of Pharmacology, UC Davis School of Medicine.
The lab of Dr. Peggy Farnham
UC Davis Genome Center Expression Analysis Core Facility
Publications
Evaluation of whole genome amplification protocols for array and oligonucleotide CGH.
Hittelman A, et al.Diagn Mol Pathol. 2007 Dec; 16(4), 198-206.
(PubMed abstract)
A novel role for the Aurora B kinase in epigenetic marking of silent chromatin in differentiated postmitotic cells.
Sabbattini P, et al.EMBO J. 2007 Nov 14; 26(22), 4657-69.
(PubMed abstract)
Comparison of sample preparation methods for ChIP-chip assays.
O'Geen H, Nicolet CM, Blahnik K, Green R, Farnham PJ. Biotechniques. 2006 Nov; 41(5): 577-80
(PubMed abstract)
Oligonucleotide Array CGH Analysis of a Robust Whole Genome Amplification Method
C. Brueck et. al., BioTechniques, Vol. 42, No. 2, 2007
(465 Kb PDF)
Webcasts
Advances in Genetic Analyis of Archived Samples
This on-demand webcast from DRUG DISCOVERY& DEVELOPMENT and G&P addresses the recent advances in Whole Genome Amplification that have made it possible to gain access to sufficient quantities of DNA from the millions of archived samples to perform a number of downstream applications including QPCR, CGH microarray, STR analysis, and SNP analysis.
White Papers
Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA) (377 Kb PDF)
Ernest Mueller, Principal Investigator, Sigma R&D
RNAlater® is a registered trademark of Ambion.
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