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Purification of Amplified Products performed with GenElute™ PCR Clean-up Kit (Product No. NA1020)
- Insert a GenElute Miniprep Binding Column (with a blue o-ring) into a provided collection tube.
- Add 0.5 ml of the Column Preparation Solution to each Miniprep column and centrifuge at 12,000 × g for 30 seconds to 1 minute. Discard the eluate.
Note: The Column Preparation Solution maximizes binding of the DNA to the membrane resulting in more consistent yields.
- Add 5 volumes of Binding Solution to 1 volume of the PCR reaction and mix.
Example: add 500 µl of Binding Solution to 100 µl of the PCR reaction.
- Transfer the solution into the binding column. Centrifuge the column at maximum speed (12,000–16,000 × g) for 1 minute. Discard the eluate, but retain the collection tube.
- Replace the binding column in the collection tube. Apply 0.5 ml of diluted Wash Solution to the column and centrifuge at maximum speed for 1 minute. Discard the eluate, but retain the collection tube.
Note: Be sure to add ethanol to the Wash Solution Concentrate prior to first time use. See Preparation Instructions.
- Replace the column in the collection tube. Centrifuge the column at maximum speed for 2 minutes, without any additional wash solution, to remove excess ethanol. Discard any residual eluate as well as the collection tube.
- Transfer the column to a fresh 2 ml collection tube. Apply 50 µl of Elution Solution or water to the center of each column. Incubate at room temperature for 1 minute.
Note: When eluting with water, make sure that the pH of the water is between 5.5 and 8.5. Elution may also be performed using the Elution Solution diluted 10-fold with water.
- To elute the DNA, centrifuge the column at maximum speed for 1 minute. The PCR amplification product is now present in the eluate and is ready for immediate use or storage at –20 °C .
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