| Three primer sets designed for use as internal controls in PCR-based Arabidopsis gene expression analysis. All three primer sets have been functionally validated and optimized by RT-PCR analysis against major Arabidopsis tissues for constitutive expression and specific amplification (see Figures 1 & 2). Further confirmation of those primers, as internal control for data normalization, was conducted in quantitative RT-PCR reactions for pathogen-inducible
Arabidopsis genes. |
These control primers provide Arabidopsis researchers a simple solution for selecting internal control(s) in gene expression analysis:
- Sensitive, efficient and cost-effective in a ready-to-use format.
- Represent different expression levels to match target expression level.
- Choice of three housekeeping genes to meet customers' specific needs.
| |
| Each set contains forward and reverse primers for housekeeping genes: |
| Product Code |
Product Name |
| C3115 |
Control Primer Set for Arabidopsis CBP20 Gene
Gene Locus: At5g44200
Amplicon Size: 409 bp |
| C3615 |
Control Primer Set for Arabidopsis Actin-2 Gene
Gene Locus: At3g18780
Amplicon Size: 257 bp |
| C3240 |
Control Primer Set for Arabidopsis UBC Gene
Gene Locus: At5g25760
Amplicon Size: 217 bp |
| Each primer set (forward and reverse) is suitable for 100 PCR/RT-PCR reactions (for 50 µL reaction volume). |
|
