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The real-time DNA extraction and amplification kit
Detailed Product Description
In the SYBR Green Extract-N-Amp PCR protocol, genomic DNA is released from leaf tissue, but never purified. This is the key difference between the Extract-N-Amp method and other genomic DNA purification kits and methods. This difference offers a tremendous time savings because it removes having to grind tissue samples, filter out cellular debris, centrifuge small columns, or alcohol precipitate the DNA.
Other methods used to prepare plant genomic DNA for PCR:
- CTAB protocols (organic extraction)
- salting out and precipitation
- kits using silica spin columns (e.g. DNeasy™)
- magnetic beads
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Because of the robustness of PCR, complete purification of the genomic DNA is unnecessary for normal functioning of the Taq polymerase. However, inhibitors to PCR are present in leaf tissue cells. The rich diversity of secondary metabolites found in the majority of plant species are particularly inhibitory. Therefore, the Extract-N-Amp protocol balances these two considerations by limiting the number of cells that are lysed. With a small lysate, sufficient full length genomic DNA is released to serve as template while inhibitors to PCR are effectively neutralized by components in the the Kit reagents.
The result of this unique protocol is the ability to stream line the process of generating genotype data from plant leaf tissue. Data results are comparable to protocols that use purified genomic DNA and the Extract-N-Amp protocol’s robustness produces reproducible data.
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