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The total RNA purification method for difficult plant tissues
Few methods are capable of delivering desired amounts of total RNA from plant tissues that contain high levels of secondary metabolites. The Spectrum Plant Total RNA Kit was designed to be different. Incorporating a new, patent-pending lysis and binding chemistry, this kit is intended to purify total RNA from species and tissue where most protocols fail. Without sacrificing affordability, you can purify more total RNA from difficult tissue samples.
Product Description [ Detailed Product Description ]
The Spectrum Plant Total RNA Kit employs a new lysis and binding chemistry and a convenient column-based 'bind-wash-and-elute' format to purify up to 100 µg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20 — 60 µg. After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants. Lastly, total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR. The Spectrum Plant Total RNA Kit contains all the reagents, buffers and solutions necessary to perform the method as described.
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