Murashige & Skoog Media

Murashige and Skoog basal salt mixture (MS)
Murashige and Skoog basal salt macronutrient solution
Murashige and Skoog basal salt micronutrient solution
Murashige and Skoog modified basal salt mixture
Murashige and Skoog modified basal salt mixture
Murashige and Skoog modified basal salt mixture
Murashige and Skoog modified basal salt mixture

Component (mg/L)	M 5524	M 0654	M 0529	M 0153	M 2909	M 8900	M 8280
Aluminum chloride • 6H₂O
Ammonium nitrate	1650.0	1650.0		825.0		825.0
Ammonium phosphate monobasic
Ammonium sulfate
Boric acid	6.2		6.2	6.2	6.2	6.2	6.2
Calcium chloride anhydrous	332.2	332.2		166.1	332.2	332.2	332.2
Calcium nitrate
Calcium phosphate tribasic
Cobalt chloride • 6H₂O	0.025		0.025	0.025	0.025	0.025	0.025
Cupric sulfate • 5H₂O	0.025		0.025	0.025	0.025	0.025	0.025
Na₂-EDTA	37.26		37.3	18.63	37.26	37.26	37.26
ZnNa₂-EDTA
Ferric chloride • 6H₂O
Ferric citrate
Ferric sulfate
Ferric tartrate • 2H₂O
Ferrous sulfate • 7H₂O	27.8		27.8	13.9		27.8	27.8
Magnesium sulfate	180.7	180.7		90.35	180.7	180.7	180.7
Manganese chloride • 4H₂O
Manganese sulfate • H₂O	16.9		16.9	16.9	16.9	16.9	16.9
Molybdenum trioxide
Molybdic acid (sodium salt) • 2H₂O	0.25		0.25	0.25	0.25	0.25	0.25
Nickel chloride • 6H₂O
Nickel sulfate • 6H₂O
Potassium chloride
Potassium iodide	0.83		0.83	0.83	0.83	0.83	0.83
Potassium nitrate	1900.0	1900.0		950.0	1900.0	950.0	1900.0
Potassium phosphate monobasic	170.0	170.0		85.0	170.0	170.0	170.0
Potassium sulfate
Sodium nitrate							1751.0
Sodium phosphate monobasic
Sodium sulfate
Zinc nitrate • 6H₂O
Zinc sulfate • 7H₂O	8.6		8.6	8.6	8.6	8.6	8.6
Organics (mg/L)	M 5524	M 0654	M 0529	M 0153	M 2909	M 8900	M 8280
Activated charcoal
Adenine hemisulfate
Agar
6-Benzylaminopurine (BA)
D-Biotin
6gamma-gamma- Dimethylallylaminopurine
Glycine (free base)
Indole-3-acetic acid
Indole-3-butyric acid
Kinetin
MES (free acid)
myo-Inositol
alpha-Naphthaleneacetic acid
Nictotinic acid (free acid)
Peptone
Pyridoxine • HCl
Sucrose
Thiamine • HCl
	M 5524	M 0654	M 0529	M 0153	M 2909	M 8900	M 8280
Grams of powder to prepare 1 L	4.3	n/a	n/a	2.2	2.7	2.6	4.4
pH 1 0.5 at room temperature	3.9	4.3	3.2	3.9	3.9	3.9	4.1
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Media including Organics

M 6899
M 5519
M 0404
M 9274
M 7024

Murashige and Skoog basal salts with minimal organics
Murashige and Skoog basal medium
Murashige and Skoog basal medium with Gamborg's vitamins
Murashige and Skoog basal medium with sucrose and agar
Murashige and Skoog shoot multiplication medium A

Component (mg/L)	M 6899	M 5519	M 0404	M 9274	M 7024
Aluminum chloride • 6H₂O
Ammonium nitrate	1650.0	1650.0	1650.0	1650.0	1650.0
Ammonium phosphate monobasic
Ammonium sulfate
Boric acid	6.2	6.2	6.2	6.2	6.2
Calcium chloride anhydrous	332.2	332.2	332.2	332.2	332.2
Calcium nitrate
Calcium phosphate tribasic
Cobalt chloride • 6H₂O	0.025	0.025	0.025	0.025	0.025
Cupric sulfate • 5H₂O	0.025	0.025	0.025	0.025	0.025
Na₂-EDTA	37.26	37.26	37.26	37.26	37.26
ZnNa₂-EDTA
Ferric chloride • 6H₂O
Ferric citrate
Ferric sulfate
Ferric tartrate • 2H₂O
Ferrous sulfate • 7H₂O	27.8	27.8	27.8	27.8	27.8
Magnesium sulfate	180.7	180.7	180.7	180.7	180.7
Manganese chloride • 4H₂O
Manganese sulfate • H₂O	16.9	16.9	16.9	16.9	16.9
Molybdenum trioxide
Molybdic acid (sodium salt) • 2H₂O	0.25	0.25	0.25	0.25	0.25
Nickel chloride • 6H₂O
Nickel sulfate • 6H₂O
Potassium chloride
Potassium iodide	0.83	0.83	0.83	0.83	0.83
Potassium nitrate	1900.0	1900.0	1900.0	1900.0	1900.0
Potassium phosphate monobasic	170.0	170.0	170.0	170.0	170.0
Potassium sulfate
Sodium nitrate
Sodium phosphate monobasic					148.0
Sodium sulfate
Zinc nitrate • 6H₂O
Zinc sulfate • 7H₂O	8.6	8.6	8.6	8.6	8.6
Organics (mg/L)	M 6899	M 5519	M 0404	M 9274	M 7024
Activated charcoal
Adenine hemisulfate					80.0
Agar				8000.0
6-Benzylaminopurine (BA)
D-Biotin
6gamma-gamma- Dimethylallylaminopurine					30.0
Glycine (free base)		2.0		2.0
Indole-3-acetic acid					0.3
Indole-3-butyric acid
Kinetin
MES (free acid)
myo-Inositol	100.0	100.0	100.0	100.0	100.0
alpha-Naphthaleneacetic acid
Nictotinic acid (free acid)		0.5	1.0	0.5
Peptone
Pyridoxine • HCl		0.5	1.0	0.5
Sucrose				30,000	30,000
Thiamine • HCl	0.4	0.1	10.0	0.1	0.4
	M 6899	M 5519	M 0404	M 9274	M 7024
Grams of powder to prepare 1 L	4.4	4.4	4.4	42.4	34.7
pH 1 0.5 at room temperature	3.9	3.9	3.8	5.0	3.9
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