Sigma offers a collection of Molecular Probes’ SYPRO stains for luminescent detection of proteins separated by PAGE. Different stains are available for 1D gel, 2D gel, and membrane detection of protein. The collection of SYPRO stains includes SYPRO Ruby, SYPRO Tangerine, SYPRO Orange, and SYPRO Red stains.
SYPRO Ruby Gel Stain
Molecular Probes' SYPRO Ruby gel stain is a ready–to–use, ultra sensitive, luminescent stain for the detection of proteins separated by PAGE. Designed especicially for 2D PAGE, SYPRO Ruby also performs impressively in PAGE and IEF gels. Ex/Em = 280, 450/610 nm
Features & Benefits
Uses a simple staining protocol with no possibility of over–staining
Delivers a linear quantitation range over three orders of magnitude
Stains glycoproteins, lipoproteins, calcium binding proteins, fibrillar proteins and others
Will not stain extraneous nucleic acids
Sensitive to 1 ng of protein
|
SYPRO Ruby protein gel stain compared to a silver stain.
Proteins from a fibroblast cell lysate were run on identical 2–D gels and stained with SYPRO Ruby protein gel stain (left) or silver stain (right). The grayscale values of the gel stained with SYPRO Ruby dye have been inverted for easier comparison with the silver–stained gel. |
| Product # |
Product Name |
Size |
| S4942 |
SYPRO Ruby Gel Stain |
1 kit |
back to top
SYPRO Tangerine Gel Stain
Molecular Probes' SYPRO Tangerine gel stain is an extremely versatile stain for proteins in SDS gels. Proteins stained without fixation can be used for zymography (in–gel enzyme activity) assays. Stained proteins can also be eluted from gels and used for further analysis. The stain does not alter protein structure and does not interfere with mass spectrometry. In addition, staining does not iterfere with the transfer of proteins to membranes, allowing visualization before preceeding with Western blotting. Ex/Em = 300, 490/640 nm
Features & Benefits
Detects 4–8 ng of protein per mini–gel band
Staining is complete in less than an hour
Selectively detects proteins without staining nucleic acids or lipopolysacharide contaminants
|
Sypro Tangerine Gel stain Zymographic detection of β–D–glucuronidase activity after visualization of total protein patterns using SYPRO Tangerine protein gel stain.
Molecular weight standards containing decreasing
amounts of Escherichia coli β–glucuronidase were run on identical SDS–polyacrylamide gels and stained with SYPRO Tangerine protein gel stain (top) followed by incubation with ELF 97 D–glucuronidase substrate to detect the activity of β–D–glucuronidase (bottom). |
| Product # |
Product Name |
Size |
| S5942 |
SYPRO Tangerine Gel Stain |
1 kit |
back to top
SYPRO Orange Gel Stain
Molecular Probes' SYPRO Orange protein gel stain provides fast, simple, sensitive staining of proteins in electrophoretic gels.
Features & Benefits
Detect 4–8 ng of protein per mini–gel band
Minimize protein–to–protein variability through increased staining consistency and eliminate negative staining
Fluorescence intensity is linear with protein quantity over three orders of magnitude, much broader than Coomassie or Silver Staining can provide
|
Protein Molecular Weight Standards separated on identical 12% SDS–PAGE gels and then stained with either SYPRO Orange (left) or SYPRO Red (right) protein gel stains. |
| Product # |
Product Name |
Size |
| S5692 |
SYPRO Orange Gel Stain |
1 kit |
back to top
SYPRO Red Gel Stain
Molecular Probes' SYPRO Red protein gel stain provides fast, simple, sensitive staining of proteins in electrophoretic gels.
Features & Benefits
Detect 4–8 ng of protein per mini–gel band
Minimize protein–to–protein variability through increased staining consistency and eliminate negative staining
Fluorescence intensity is linear with protein quantity over three orders of magnitude, much broader than Coomassie or Silver Staining can provide
See above gel
| Product # |
Product Name |
Size |
| S5817 |
SYPRO Red Gel Stain |
1 kit |
back to top
SYPRO Protein Gel Starter Kit
The SYPRO gel starter kit includes three fluorescent protein stains (SYPRO Orange, SYPRO Red, and SYPRO Tangerine) as well as a SYPRO photographic filter. Proteins as small as 6.5 kDa can be detected with no observed staining of liposaccharides or nucleic acids.
| Product # |
Product Name |
Size |
| SY0100 |
SYPRO Protein Gel Starter Kit |
1 kit |
SYPRO is a registered trademark of Molecular Probes.
back to top
back to Protein Electrophoresis
|
