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We offer several stains designed for use in Western blot analysis. These stains include Amido Black, Ponceau S, Alcian Blue and many more.
Alcian Blue 8GX, A 3157
Alcian Blue 8GX is suitable for detection of glycoproteins on nitrocellulose and in PAGE gels. Alcian Blue 8GX reacts with sulfate and carboxylated functional groups. The interaction of Alcian Blue (cationic) and polyanionic glycoproteins is influenced by pH; both sulfate and carboxylate groups will react at pH 2.5, but sulfate groups alone react at pH 1. Available in 10 g and 25 g sizes.
Amido Black Staining Solution, 2x concentrate, A8181
Amido Black Staining Solution is designed for rapid staining of protein bands on nitrocellulose membranes. Amido Black Staining Solution facilitates visualization of low concentration proteins with a low background. Proteins can be easily destained with 25% (v/v) isopropanol, and 10% (v/v) acetic acid for further analysis. PAGE gels. Each bottle of 2x concentrate, dilutes to 500 mL with water.
EZBlue™ Gel Staining Reagent, G1041
Convenient, sensitive, and safe, EZBlue Coomassie Brilliant Blue G-250 colloidal protein stain improves protein electrophoresis results while significantly reducing staining time. Conveniently packaged, EZBlue requires no messy weigh-ups or additions of methanol or acid. As a colloidal stain, it reacts only with proteins, not the gel itself. Background staining is reduced, so protein bands can be visualized almost immediately. No destaining step is required, although a water wash may intensify bands and clarify the background. EZBlue is compatible with both PAGE gels and PVDF membranes. Most impressively, EZBlue is extremely sensitive, detecting as little as 5 ng of protein. Available in 500 mL and 3.8 L sizes.
Fast Green FCF, F7252
Fast Green FCF is used for protein detection and quantitation in native PAGE, SDS-PAGE, and IEF gels. Following staining, protein concentration can be measured at 625 nm. Fast Green staining is linear over a wider range of detection than Brilliant Blue R. It is especially well suited to IEF because it does not bind to ampholytes. Available in 5 g and 25 g sizes.
Gold, Colloidal, 50755
As a widely used protein and polysaccharide marker for electron microscopy, colloidal gold is a useful general stain for proteins immobilized on nitrocellulose membranes following PAGE. Available in 500 mL size.
Glycoprotein Detection Kit, GLYCO-PRO
The glycoprotein detection kit is designed to selectively stain glycoproteins in polyacrylamide gels and membranes using a modification of the Periodic Acid-Schiff (PAS) methods. Staining of sugar moieties of glycoproteins yields magenta bands with a colorless background. The detection limits using this kit are 25-100 ng of carbohydrate per band. Each
kit contains sufficient materials for 10 mini-gels (10 x 10 cm).
Ponceau S, P3504
Ponceau S (sodium salt) may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or PVDF membranes (Western blotting), as well as on cellulose
acetate membranes. Common stain formulations include 0.1% (w/v) Ponceau S in 5% acetic acid or 2% (w/v) Ponceau S in 30% TCA and 30% sulfosalicylic acid. Available in 10 g, 50 g and 100 g sizes.
Ponceau S Solution, P7170
Ponceau S Solution is a ready-to-use, reversible staining solution designed for rapid (5 minute) staining of protein bands on nitrocellulose or PVDF membranes (Western blots), as well as cellulose acetate membranes. Ponceau S stain is easily reversed with water washes, facilitating subsequent immunological detection. Available in 1 L size.
Reversible Protein Detection Kit
for Membranes and Polyacrylamide Gels, R-PROB
The Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels is a unique protein detection product for visualization of proteins on nylon, nitrocellulose, and PVDF membranes, as well as PAGE gels with sensitivity comparable to that of Coomassie stains. Many other stains produce high backgrounds on nylon membranes due to strong charge interactions. Application of activated R-PROB produces lavender-stained protein bands, which are easily destained (reversed) with an EDTA solution. Each kit is sufficient for 200 applications (small blots on 8 x 10 cm PAGE gels).
SYPRO® Ruby Protein Blot Stain, S4817
Fluorescent SYPRO Ruby protein blot stain provides a rapid, simple, and highly sensitive method for detecting proteins on nitrocellulose or polyvinylidene difluoride (PVDF) membranes
(blots). The bright orange-red fluorescent stain can be easily visualized using UV illumination or a laser scanner. Available in 200 mL sizes.
SYPRO is a registered trademark of Molecular Probes.
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