Further optimization of preliminary crystallization conditions is often achieved by addition of traces of small molecules. Although the mechanisms are often not investigated or understood, additives seem to manipulate sample-solvent or sample-sample interactions or the structure of solvent (water). They may stabilize or increase conformity of crystals by specific interactions with biological macromolecules.
The additive kit provides 48 different solutions of various additives, including organic solvents, salts, amino acids and other compounds. This kit provides an easy tool for rapid screening of additives. The kit includes 100 µl of each pre-formulated compound (solutions of organic solvent 1 mL).
Similar to the additive kit this kit provides a range of detergents that may be used for optimization. Detergents often interfere with the crystallization process. They are used to influence hydrophobic sample-sample interactions that may cause non-specific aggregation. On the other hand they can also influence the structure of water. The use of detergents is typically required for crystallization of membrane proteins or other proteins, if non-specific aggregation is suspected.
The detergent kit includes 48 defined detergents that are pre-formulated for ease of use. The concentration of each detergent solution is adjusted to the 10-fold value of critical micelle concentration. The kit includes 100 µl of each pre-formulated detergent.
| Kit Compound No. |
Product # |
Additive |
| 1 |
61941 |
Ethylene glycol 5 M |
| 2 |
38262 |
2-Methyl-2,4-pentanediol 3 M |
| 3 |
50447 |
(+/-) -1,3-Butanediol 4 M |
| 4 |
68891 |
1,4-Butanediol 4 M |
| 5 |
28428 |
1,6 Hexanediol 3 M |
| 6 |
50788 |
2,5 Hexanediol 3 M |
| 7 |
39206 |
1,2,3-Hexanetriol 1 M |
| 8 |
50791 |
1,2,3-Heptanetriol Isomer H 1 M |
| 9 |
67190 |
1,2,3-Heptanetriol Isomer T 1 M |
| 10 |
56592 |
1,2,3-Octanetriol Isomer H 1 M |
| 11 |
97596 |
1,2,3-Octanetriol Isomer T 1 M |
| 12 |
50722 |
Ammonium sulfate 1 M |
| 13 |
43568 |
Cesium chloride 1 M |
| 14 |
44675 |
Potassium chloride 1 M |
| 15 |
40113 |
Lithium chloride 1 M |
| 16 |
16818 |
Sodium chloride 2 M |
| 17 |
08536 |
Natrium thiocyanate 2 M |
| 18 |
08756 |
Sodium iodide 1 M |
| 19 |
67414 |
Sodium fluoride 0.5 M |
| 20 |
55298 |
Barium chloride 0.1 M |
| 21 |
53704 |
Cadmium chloride 0.1 M |
| 22 |
00457 |
Calcium chloride 0.1 M |
| 23 |
28811 |
Cobalt chloride 0.1 M |
| 24 |
15862 |
Cu(II)-chloride 0.1 M |
| 25 |
18865 |
Magnesium chloride 0.1 M |
| 26 |
48795 |
Manganese(II)-chloride 0.1 M |
| 27 |
69042 |
Strontium chloride 0.1 M |
| 28 |
28962 |
Yttrium chloride 0.1 M |
| 29 |
39059 |
Zinc chloride 0.1 M |
| 30 |
05292 |
Spermidine 0.1 M |
| 31 |
28481 |
Spermine tetrahydrochloride 0.1 M |
| 32 |
08740 |
6-Aminohexanoic acid 1 M |
| 33 |
08730 |
1,5-Diaminopentanedihydrochloride 1 M |
| 34 |
67258 |
1,6-Diaminohexane 1 M |
| 35 |
16426 |
1,8-Diaminooctane 1 M |
| 36 |
11102 |
3-(1-Pyridinio)-1-propanesulfonate 2 M |
| 37 |
63226 |
Benzamidinhydrochloride 1 M |
| 38 |
49541 |
Xylitol 1 M |
| 39 |
02724 |
Taurine 0.1 M |
| 40 |
18472 |
βine 0.1 M |
| 41 |
64961 |
Trimethylamine hydrochloride 0.1 M |
| 42 |
50535 |
Hexamminecobalt trichloride 0.1 M |
| 43 |
18252 |
Ethylenediamine tetracetic acid Sodium salt 0.1 M |
| 44 |
14495 |
L-Cysteine 0.1 M |
| 45 |
08591 |
Sodium azide 0.1 M |
| 46 |
12146 |
Glycerol 3 M |
| 47 |
67419 |
Glycine 1 M |
| 48 |
07458 |
Glycyl-glycyl-glycine 0.3 M |
| Kit Compound No. |
Product # |
Detergent |
| 1 |
50006 |
Hexyl-β-D-glucoside 200 mM |
| 2 |
44615 |
Heptyl-β-D-glucoside 150 mM |
| 3 |
18842 |
Octyl-β-D-glucoside 100 mM |
| 4 |
30887 |
Octyl-α/β-glucoside 100 mM |
| 5 |
11448 |
Nonyl-β-D-glucoside 50 mM |
| 6 |
04978 |
Methyl-6-O-(N-heptylcarbamoyl)-α-D-glucopyranoside 200 mM |
| 7 |
42387 |
Triethyleneglycol monoocyl ether 1 mM |
| 8 |
41957 |
Triethyleneglycol monodecyl ether 1 mM |
| 9 |
07891 |
Triethyleneglycol monododecyl ether 1 mM |
| 10 |
13172 |
Tetraethylenglykolmonooctylether 1 mM |
| 11 |
07888 |
Tetraethyleneglycol monodecyl ether 1 mM |
| 12 |
16802 |
Tetraethyleneglycol monododecyl ether 1 mM |
| 13 |
28000 |
Pentaethyleneglycol monodecyl ether 1 mM |
| 14 |
04556 |
Pentaethyleneglycol monododecyl ether 1 mM |
| 15 |
03269 |
Hexaethyleneglycol monodecyl ether 1 mM |
| 16 |
15931 |
Hexaethyleneglycol monododecyl ether 1 mM |
| 17 |
39376 |
Heptaethyleneglycol monodecyl ether 1 mM |
| 18 |
49534 |
Heptaethyleneglycol monododecyl ether 1 mM |
| 19 |
07511 |
Octaethyleneglycol monodecyl ether 1 mM |
| 20 |
55377 |
Octaethyleneglycol monododecyl ether 1 mM |
| 21 |
40913 |
Nonaethyleneglycol monododecyl ether 1 mM |
| 22 |
07509 |
Decyl-β-D-maltoside 10 mM |
| 23 |
04836 |
Undecyl-β-D-maltoside 10 mM |
| 24 |
54202 |
Dodecyl-β-D-maltoside 3 mM |
| 25 |
08386 |
Hexadecyl-β-D-maltoside 0.1 mM |
| 26 |
54280 |
Cyclohexylmethyl-β-D-maltoside 500 mM |
| 27 |
19164 |
2-Cyclohexylethyl-β-D-maltoside 200 mM |
| 28 |
41583 |
6-Cyclohexylpentyl-β-D-maltoside 30 mM |
| 29 |
51793 |
6-Cyclohexylhexyl-β-D-maltoside 30 mM |
| 30 |
39489 |
Heptyl-β-D-thioglucoside 300 mM |
| 31 |
07818 |
Nonyl-β-D-1-thiomaltopyranoside 100 mM |
| 32 |
38498 |
Decyl-β-D-1-thiomaltopyranoside 100 mM |
| 33 |
19899 |
CHAPS 100 mM |
| 34 |
39490 |
MEGA-8 500 mM |
| 35 |
44882 |
MEGA-9 200 mM |
| 36 |
43104 |
N,N-Dimethylhexylamine N-oxide 100 mM |
| 37 |
41261 |
N,N-Dimethyloctylamine N-oxide 100 mM |
| 38 |
49556 |
N,N-Dimethylnonylamine N-oxide 100 mM |
| 39 |
18705 |
N,N-Dimethyldecylamine N-oxide 100 mM |
| 40 |
57915 |
N,N-Dimethyldodecylamine N-oxide 100 mM |
| 41 |
54185 |
3-(N,N-Dimethyloctylammonio)propanesulfonate 500 mM |
| 42 |
54196 |
3-(N,N-Dimethyldecylammonio)propanesulfonate 300 mM |
| 43 |
16974 |
3-(N,N-Dimethyldodecylammonio)propanesulfonate 30 mM |
| 44 |
53502 |
3-(N,N-Dimethylmyristoylammonio)propanesulfonate 3 mM |
| 45 |
44490 |
3-(N,N-Dimethylpalmitoylammonio)propanesulfonate 0.3 mM |
| 46 |
|
O-(n-Octyl)-phosphorylcholine 1 M |
| 47 |
|
O-(n-Decyl)-phosphorylcholine 100 mM |
| 48 |
|
O-(n-Tetradecyl)-phosphorylcholine 1.5 M |
