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Crystallization Kits | Crystallization Plates | Membrane Protein Kit | Optimization Kits | Optimization Reagents | Renaturation Kit

Renaturation Basic Kit for Proteins

The Renaturation Basic Kit for Proteins is a rapid empirical screening method used to determine the best conditions for the renaturation of proteins that have been solubilized from inclusion bodies. It is known that physical parameters, such as pH, redox potential, ionic strength, low-molecular weight additives and temperature, may have an effect on the efficiency of in vitro renaturation processes. Screening with this kit is an easy method to find renaturation conditions in a defined pH range of 6.5 to 8.5, defined redox potential and ionic strength conditions, as well as the effect of one sulfobetaine, namely 3-(1-1-pyridinio)-1-propanesulfonate. Other parameters, e.g., concentration of the solubilized protein, temperature and additional additives, can easily be added to the basic screening conditions of this kit.

Overexpression of proteins in prokaryotes often leads to the production of insoluble aggregates of misfolded proteins in inclusion bodies. Often considered a nuisance, the formation of inclusion bodies has the advantage of a high enrichment of the desired protein at an early stage of purification. Furthermore, the recombinant protein is protected in inclusion bodies against proteolysis by intracellular proteases. These inclusion bodies can easily be purified and may be the best method for the production of proteins that are lethal to the host cells. However, the solubilization of the expressed protein can only be obtained using strongly denaturing conditions. The major task is to achieve an efficient in vitro renaturation to the properly folded protein.

Helpful Notes:

Renaturation Kit Datasheet including composition (226 kb PDF)

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