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CelLytic IB was designed to solubilize protein aggregates called inclusion bodies
(Figure 1). In bacteria, inclusion bodies are sometimes formed when recombinant proteins are overexpressed. CelLytic IB was formulated to solubilize the protein of interest for immediate analysis of protein content or refolding procedures.
| Product # |
Product Name |
Package Size |
|
C5236 |
CelLytic IB Cell Lysis Reagent |
25 ml
100 ml |
|
Figure 1. Solubilization of Streptavidin Inclusion Bodies.
Samples of solubilized inclusion body protein were assayed using BCA Reagent (BCA-1). 50 µl of each sample was incubated with BCA reagent at 60 °C for 15 min. The samples were then cooled to room temperature and assayed at 562 nm. The data above has been standardized to the protein recovery of CelLytic IB. |
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