CelLytic Nu-CLEAR Extraction Kit

Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cell. A number of different procedures in the detailed technical bulletin enable the selection that best fits a particular application. For example, choose between detergent and non-detergent extraction of nuclear protein or between the standard hypotonic lysis buffer for most cell types and isotonic lysis buffer for fragile cells. In addition, the kit provides a procedure for salt reduction from the nuclear extract with dilution buffer. CelLytic Nu-CLEAR offers the flexibility you need for optimal protein extraction. Extracts can be prepared in less than 2 hours and are highly pure since there is little or no cross-contamination between nuclear and cytoplasmic extracts (Figure 1). Each kit is sufficient for 100 extractions of 100 µl of packed cell volume.

Figure 1. Purity of Cytoplasmic and Nuclear Proteins. The double stranded 32P-labeled Octamer motif oligonucleotide was incubated with either cytoplasmic fraction (C) or nuclear extract (N) prepared from HeLa, CHO, COS and PC-12 cells using the CelLytic Nu-CLEAR extraction kit.

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