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CelLytic™ P is a non-ionic detergent-based reagent that offers a convenient method for efficient plant cell lysis and protein solubilization. It is a non-denaturing reagent and maintains protein immunoreactivity and biological activity (Figure 1). CelLytic P is efficient, rapid, and ready to use. It contains bicine buffer, which is preferable for many biological activities. Use of CelLytic P enables extraction of proteins from less than one gram to hundreds of grams of fresh or frozen leaves, employing the same
short procedure. It has been tested on leaves from four plant models: tobacco, tomato, spinach, and arabidopsis.
| Product # |
Product Name |
Package Size |
|
C 2360 |
CelLytic P Cell Lysis Reagent |
50 ml
250 ml |
|
Figure 1. Compatibility with Gel Shift Assay.
Protein extracts were prepared with the CelLytic P reagent from spinach leaves. A double stranded 32P labeled CREB oligonucleotide probe was incubated with 28 µg of the
whole cell extract (lanes 2-5) or without whole cell extract (lane 1, free probe). Binding reactions with the extracts were performed in the absence [–] of competitor oligonucleotide
(lanes 1-2) or in the presence of 100 or 500 fold excess of unlabeled CREB binding motif oligonucleotide (specific competitor [SP], lane 3 and lane 4, respectively) or in the presence
of 100 fold excess of unlabeled oligonucleotide (non specific competitor, [NS] lane 5). Binding reactions were run on a non-denaturing 6% polyacrylamide gel, dried, and imaged on X-ray film. The arrows indicate the CREB-DNA complex and the free probe. |
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