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The following Cold Spring Harbor Laboratory Press protocols are brought to you by BioSupplyNet in a partnership with Sigma-Aldrich. A list of products is available to use in each protocol.
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Selected Protocol from "Protein-Protein Interactions", 2nd Edition, E. Golemis, P. Adam.
Digestion of Proteins and Extraction of Peptides from an Acrylamide Gel
Sherry Niessen, Ian McLeod and John R. Yates III
Department of Cell Biology, The Scripps Research Institute, La Jolla, California
ABSTRACT
Analysis of cellular proteins by Mass Spectrometry requires the isolation of protein complexes, and enzymatic digestion into peptides. After proteins are separated into bands through electrophoresis on an acrylamide gel, this protocol provides a method for reduction, alkylation and in-gel digestion into peptides, as well as extraction of the resultant peptides. |
Buy this book, Z719145
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Products Available for this Protocol
| Protocol Material Description |
Product # |
Product Name |
Add to Cart |
| Ammonium bicarbonate, 100mM |
A6141 |
Ammonium bicarbonate |
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| CaCl2, 1 M |
21115 |
Calcium chloride solution |
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| Formic Acid, 90%, 5% |
F0507 |
Formic acid |
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| Iodoacetamide (IAA), 55 mM |
I1149 |
Iodoacetamide |
|
| K3Fe(CN)6 |
P8131 |
Potassium hexacyanoferrate(III) |
|
| Na2S2O3-5H2O |
S6672 |
Sodium thiosulfate pentahydrate |
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| Tris (2-carboxyethyl)-phosphine hydrochloride (TCEP) |
C4706 |
Tris(2-carboxyethyl)phosphine hydrochloride |
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| Trypsin, seq. grade, modified |
T8658 |
Trypsin from bovine pancreas |
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Product Association Disclaimer:
The Sigma-Aldrich products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. The products/reagents from Sigma-Aldrich have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest.
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