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The following Cold Spring Harbor Laboratory Press protocols are brought to you by BioSupplyNet in a partnership with Sigma-Aldrich. A list of products is available to use in each protocol.
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Selected Protocol from "Protein-Protein Interactions", 2nd Edition, E. Golemis, P.
Adam.
Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
Margret B. Einarson and Elena N. Pugacheva
Foxx Chase Cancer Center, Philadelphia, PA 19111
Jason R. Orlinck
Brigham & Women’s Hospital, Boston, MA 02115
ABSTRACT
Glutathione-S-Transferase (GST) fusion proteins have a wide range of
applications. This protocol is designed for IPTG-inducible bacterial expression
vectors. |
Buy this book, Z719145
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Products Available for this Protocol
| Protocol Material Description |
Product # |
Product Name |
Add to Cart |
| Reduced glutathione, 20 mM prepared in 50 mM Tris-HCl (pH 8.0) |
G4251 |
L-Glutathione reduced |
|
| Isopropyl-β-D-thio-galactoside (IPTG) |
I5502 |
Isopropyl β-D-1-thiogalactopyranoside |
|
| LB broth, containing appropriate antibiotic selection |
L2542 |
LB Broth |
|
| PBS |
P5368 |
Phosphate buffered saline |
|
| 1% Triton X-100 |
X-100 |
Triton® X-100 |
|
| Aprotinin |
A1153 |
Aprotinin from bovine lung |
|
| Leupeptin |
L9783 |
Leupeptin hydrochloride |
|
| Phenylmethylsulfonyl fluoride [PMSF] |
P7626 |
Phenylmethanesulfonyl fluoride |
|
| 150 mM NaCl |
S3014 |
Sodium chloride |
|
| 20 mM sodium phosphate (pH 7.4) |
S3264 |
Sodium phosphate dibasic |
|
| Glutathione-Sepharose beads |
G4510 |
Glutathione−Agarose |
|
| Coomassie blue (see Sambrook and Russell 2001) |
B5133 |
Brilliant Blue G |
|
| 100 mM Tris-HCl (pH 6.8) |
T3253 |
Trizma® hydrochloride |
|
| 4%(w/v) SDS (electrophoresis grade) |
L4390 |
Sodium dodecyl sulfate |
|
| 0.2% (w/v) bromophenol blue |
B0126 |
Bromophenol Blue |
|
| 20% (v/v) glycerol |
G9012 |
Glycerol |
|
| 200 mM dithiothreitol (DTT) |
D9779 |
DL-Dithiothreitol |
|
| β-mercaptoethanol |
M3148 |
2-Mercaptoethanol |
|
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Product Association Disclaimer:
The Sigma-Aldrich products listed for this specific protocol were selected either to match or
to supplement the products listed within the actual protocol. The products/reagents from Sigma-Aldrich have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest.
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