EZview™ Red - High Visibility Affinity Gel

A major disadvantage of affinity-based molecular pull-down and immunoprecipitation procedures is that the affinity matrix is difficult to see in the microcentrifuge tube following centrifugation steps.

To facilitate more speed and accuracy, Sigma has developed a dye-conjugated agarose for our EZview Red Affinity Gels. The vivid red color of the affinity beads provides high visibility that allows easy differentiation of the pellet from the supernatant, therefore reducing the risk of accidental aspiration of the pellet and allowing for less tedious manipulations.

Technical Poster:
Affinity Capture Resins With Enhanced Visibility (80 kb PDF)

Data:

Figure 1. EZview Red Protein A Affinity Gel (left, Product Code: P6486) has enhanced visibility compared to standard, non-colored Protein A Agarose (right, Product Code: P2545)

Figure 2. EZview Red Protein Affinity Gel (Product Code: P2545) recovered a similar amount IgG. Different amounts (50 ug, 12.4 ug and 2.5 ug) of ANTI-FLAG® M2 Monoclonal Antibody (Product Code: F3165) were spiked into COS-7 lysates (107 cells in 1 ml RIPA buffer). The mouse IgG was recovered using standard protein A agarose (White, lanes 1-3) or EZview™Red Protein A Affinity Gel (Red, lanes 4-6) in a mock IP experiment. After washing, the bound proteins were eluted and subjected to SDS-PAGE. The gels was stained with colloidal blue stain (EZBlue™ Gel Staining Reagent, Product Code: G1041).

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