Designed for use with LC instruments, the ProteoPrep® 20 LC column accommodates a sample load of 100 µL plasma or serum and can be used up to 100 times.
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The ProteoPrep® 20 technology offers the highest depletion of highly abundant plasma proteins available today and specifically removes 20 highly abundant proteins (representing 97% of the proteome) to reveal a new layer of the plasma proteome.
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| The ProteoPrep® 20 Technology depletes the following proteins: |
Albumin
IgGs
Transferrin
Fibrinogen
IgAs
α-2-Antitrypsin
IgMs
α-1-Antitrypsin
Complement C3
Haptoglobulin |
Apolipoprotein A1
Apolipoprotein A2
Apolipoprotein B
Acid-1-Glycoprotein
Ceruloplasmin
Complement C4
Complement C1q
IgDs
Prealbumin
Plasminogen |
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Twenty-two Unique Proteins Revealed with ProteoPrep® 20 |
The ProteoPrep® 20 Plasma Immunodepletion Kit specifically removes 20 highly abundant proteins (representing 97% of the proteome) at 99% efficiency, revealing a new layer of highly abundant proteins. Initially, the gel image above appears to be similar to an undepleted sample, but identifications via mass spectrometry indicate that the visible proteins are not members of the list of twenty high abundance proteins specifically removed. They are, indeed, unique to a sample depleted with the ProteoPrep® 20 technology. |
| Spot No. |
Protein Identification |
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22 |
Alpha-1-B-glycoprotein
Hemopexin (Beta-1B-glycoprotein)
Alpha-2-HS-glycoprotein
(Fetuin A)
Vitamin D Binding Protein
Pigment Epithelium-derived factor
Carboxypeptidase N
(Kininase 1)
Complement Factor H fragment
Apolipoprotein E
Ficolin 3 (Hakata antigen)
PK-120 (Plasma Kallikrein-sensitive Glycoprotein)
Apolipoprotein A-IV
Glutathione Peroxidase 3
Tetranectin (Plasminogen Binding Protein)
Serum amyloid A (SAA)
Kininogen
Complement Factor H-related protein 2
Inter-alpha-trypsin inhibitor H1
Vitronectin
Geltain-Binding Protein
Antithrombin III
Cluterin (Apolipoprotein J)
Inter-alpha-trypsin inhibitor H4 |
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