Coomassie Stains

We offer a selection of Coomassie™ protein stains suitable for a variety of electrophoresis procedures. Coomassie blue Stains EZ Blue and Brilliant Blue are designed specifically for staining proteins in SDS-PAGE gels. Our premixed EZ blue gel-staining reagent can detect down to 5ng of protein and elminates hazardous waste disposal. The solvent based Brilliant Blue stains eliminate time consuming fixing steps, and our Coomassie™ violet R 200 is designed for rapid detection of proteins in IEF. For quality detection Sigma provides a Coomassie stain to meet all of your needs.

• EZBlue™ Gel Staining Reagent
• Brilliant Blue G Concentrate
• Brilliant Blue R Staining Solution
• Coomassie™ Violet R 200

Convenient, sensitive, and safe, EZBlue Coomassie Brilliant Blue G-250 colloidal protein stain improves protein electrophoresis results while significantly reducing staining time. Conveniently packaged, EZBlue requires no messy weigh-ups or additions of methanol or acid. As a colloidal stain, it reacts only with proteins, not the gel itself. Background staining is reduced, so protein bands can be visualized almost immediately. No destaining step is required, although a water wash may intensify bands and clarify the background. Most impressively, EZBlue is extremely sensitive, detecting as little as 5 ng of protein.

Features & Benefits

• Pre-mixed solution eliminates the time and effort required to prepare the stain
• Increased sensitivity ensures low abundance proteins can be detected (as little as 5 ng)
• Rapid reaction significantly reduces the amount of time required to stain and rinse
• Eliminates solvent waste so you save the expense of hazardous material disposal

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Brilliant Blue G Concentrate is a Coomassie G-250 methanol-based stain designed for protein detection in polyacrylamide and agarose gels. Because this stain contains methanol and acetic acid, gels require no fixing step prior to staining. Each bottle dilutes to 1 L with water.

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Brilliant Blue R Staining Solution is designed specifically for staining protein in SDS-PAGE and agarose gels. Because this stain contains ethanol and acetic acid, gels require no fixing step prior to staining. The gel is immersed in staining solution for 30 minutes and destained with 10% acetic acid.

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Coomassie Violet R 200 is used for rapid detection of proteins in IEF without removal of carrier ampholytes.

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