Sigma-Aldrich
Life Science

Antibody Explorer
Automation
Cancer Research
Cell Culture
Cell Signaling and Neuroscience
Custom Synthesis
Drug Discovery
Functional Genomics and RNAi
Metabolomics
Molecular Biology
Nutrition Research
Obesity Research
Plant Biotechnology
Proteomics and Protein Expr.
Proteomics
Protein Expression
Cloning and Expression
Cell Lysis
Protocols
Purification and Detection
Protein Analysis
Stable Isotopes
Your Favorite Gene - Search
Life Science Quarterly
PathFinder

 HIS-Select™ Supporting Products & Reagents

Purification & Detection
 
  Products for UpStream Processes
Products for DownStream Processes
Reagents and Products for use with HIS-Select™


Products for UpStream Processes  
Product Name Product # 
Cell Lysis Products  
CelLyticä B Plus Kit, Lyse 50 grams of fresh or frozen cell paste CB0500
CelLyticä B Plus Kit, Lyse up to 5 grams of fresh or frozen cell paste CB0050
CelLyticä B Bacterial Cell Lysis/Extraction Reagent B3553
CelLytic™ B-II Bacterial Cell Lysis/Extraction Reagent B3678
CelLytic™-Y Yeast Cell Lysis/Extraction Reagent C4482
CelLytic™-M Mammalian Cell Lysis/Extraction Reagent C2978
CelLytic™ MT Mammalian Tissue Lysis/Extraction Reagent
C3228
CelLytic™ P Plant Cell Lysis/Extraction Reagent C2360
CelLytic™ PN Extraction Kit CelLyt-PN-1
CelLytic™ IB Inclusion Body Solubilization Reagent C5236
CelLyticä NuCLEARä Extraction Kit N-XTRACT
Protease Inhibitor Cocktails  
Bacterial Protease Inhibitor Cocktail P8465
Mammalian Protease Inhibitor Cocktail P8340
Fungal/Yeast Protease Inhibitor Cocktail P8215
Plant Protease Inhibitor Cocktail P9599
Poly (Histidine)-tagged Proteins Protease Inhibitor Cocktail P8849
General Use Protease Inhibitor Cocktail P2714
Phosphatase Inhibitor Cocktail I P2850
Phosphatase Inhibitor Cocktail II P5726
Competent Cells for Protein Expression  
BL21 Competent Cells Uni-Pack B8808
BL21(DE3) Competent Cells Uni-Pack B8683
BL21(DE3) PLYSS Competent Cells Uni-Pack B8933
BL21(DE3) PLYSE Competent Cells Uni-Pack B9058
Prepared Escherichia Coli Media  
LB Agar, Powder L2897
LB Agar EZMixä Powder L7533
LB Agar, Tablet L7025
S-Galä/LB Agar Blend C4478
LB Broth, Powder L3022
LB Broth EZMixä Powder L7658
LB Broth, Tablet L7275
Luria Agar L3147
Luria Agar Base (Miller's Modification) L3272
Luria Broth L3522
Luria Broth (Miller's Modification) L3397
NZ Broth N3518
NZCYM Broth, Powder N3643
NZCYM Broth EZMixä Powder N6905
M9 Minimal Salts, 5X M6030
Hanahan's Broth H8032
SOC Medium S1797
Terrific Broth, modified, Powder T0918
Terrific Broth, Modified EZMixä Powder T9179
2X YT Microbial Medium, Powder Y2377
2X YT Microbial Medium EZMixä Powder Y2627
Prepared Yeast Media  
YPD Agar Y1500
YPD Broth Y1375
Yeast Nitrogen Base With Amino Acids Y1250
Yeast Nitrogen Base without amino acids and ammonium sulfate Y1251
Yeast Nitrogen Base without amino acids Y0626
Yeast Synthetic Drop-out Medium Supplement Without Histidine Y1751
Yeast Synthetic Drop-out Medium Supplement Without Leucine Y1376
Yeast Synthetic Drop-out Medium Supplement Without Tryptophan Y1876
Yeast Synthetic Drop-out Medium Supplement Without Leucine and Tryptophan Y0750
Yeast Synthetic Drop-out Medium Supplement Without Uracil Y1501
Yeast Synthetic Drop-out Medium Supplement Without Histidine, Leucine, Tryptophan and Uracil Y2001
Products for DownStream Processes back to top
Product Name Product # 
Bradford Reagent B6916
Bicinchoninic Acid Kit for Protein Determination BCA-1
Protein Standards, Micro Standard, Liquid P0914
EZBlueä Gel Staining Reagent G1041
ProteoQwestä Chemiluminescent Western Blotting Kit, CPS Substrate, (for Mouse Monoclonal IgG Antibodies) PQ0201
ProteoQwestä Colorimetric Western Blotting Kit, TMB Substrate, (for Mouse Monoclonal IgG Antibodies) PQ0101
ProteoSilverä Silver Stain Kit PROT-SIL1
ProteoSilverä Plus Silver Stain Kit PROT-SIL2
ProteoMassä Peptide and Protein MALDI-MS Calibration Kit MS-CAL1
ProteoMassä Peptide MALDI-MS Calibration Kit MS-CAL2
ProteoMassä Protein MALDI-MS Calibration Kit MS-CAL3
Trypsin solution from porcine pancreas, Proteomics Grade T6567
ProteoProfileä Trypsin In-gel Digest Kit PP0100
Reagents and Products for use with HIS-Select™ back to top
Product Name Product # 
Polypropylene Chromatography Columns, General Purpose, Size: 10 mL, Overall : 5 in. C2103
Polypropylene Chromatography Columns, General Purpose, Size: 13 mL, Overall : 7 in. C2353
HIS-Select™ Elution Buffer H5413
HIS-Select™ Wash Buffer H5288
HIS-Select™ Wash & Elution Buffer Kit HS0100
Ethylenediaminetetraacetic acid tetrasodium salt tetrahydrate, Practical Grade, approx. 95% ED4S
Ethyl alcohol, HPLC grade, denatured 27,074-1
Glycerol anhydrous, for molecular biology, minimum 99% G5516
Guanidine hydrochloride, for molecular biology, minimum 99% G3272
L-Histidine monohydrochloride monohydrate, minimum 99% (TLC) H8000
Igepal CA-630, Viscous liquid I3021
Imidazole, approx. 99%, Crystalline I0125
Iminodiacetic acid agarose, Saline suspension I4758
2-Mercaptoethanol M6250
Nickel(II) sulfate hexahydrate, Crystalline N4882
Sodium chloride, for molecular biology, minimum 98% (titration) S3014
Sodium phosphate monobasic anhydrous, minimum 99.0% S0751
Trichloroacetic acid solution, 6.1 N T0699
Triton® X-100, SigmaUltra T9284
TWEEN® 20, for molecular biology, Viscous liquid P9416
Urea, minimum 99.5%, Pellets U1250

back to top
Back to HIS-Select™ Family Page