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The E-DEGLY Kit contains all the enzymes and reagents needed to completely remove all N-linked and simple
O-linked carbohydrates from glycoproteins, as well as cleave complex core O-linked
carbohydrates including those containing polylactosamine.
Features & Benefits
- Single reaction at neutral pH
No need to adjust conditions for each glycosidase reaction
- Native & denaturing procedures
Allows flexibility to ensure resulting deglycosylated proteins are compatible with
down-stream analysis
- Uses highly purified glycosidases, free of contaminating proteases
No degradation of protein results in clear, reliable results
- Removes all N-linked and O-linked sugars containing polysialic acid
Results in more complete deglycosylation
- Includes fetuin which has sialylated N- and O-linked oligosaccharides
Provides a control to check for efficiency of
the deglycosylation
- Each reaction deglycosylates 200 µg of glycoprotein
Yields enough deglycosylated protein for further analysis
- Contains sufficient reagent for the deglycosylation of approx. 2 mg of protein
Allows for the processing of multiple samples
Kit Components
PNGase F
Endo-O-Glycosidase
a-2(3,6,8,9)-Neuraminidase (Sialidase A)
ß(1,4)-Galactosidase
ß-N-Acetylglycosaminidase
5X Reaction Buffer
Denaturation Solution
Triton™ X-100 (15% solution)
Fetuin Control
In contrast to the In-gel Deglycosylation Kit, this
kit is optimized for the removal of glycans from proteins in solution. This
can simplify electrophoretic analysis by reducing the heterogeneity normally
associated with glycoproteins. The kit is also useful if the protein(s)
of interest are not separated by electrophoresis but analyzed by other means.
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