| Modification |
Monoisotopic
Mass Change |
Average Mass
Change |
| Homeserine formed from Met by CNBR treatment |
-29.99281 |
-30.0935 |
| Pyroglutamic acid formed from Gln |
-17.02655 |
-17.0306 |
| Disulphide bond formation |
-2.01565 |
-2.0159 |
| C-terminal amide formed from Gly |
-0.98402 |
-0.9847 |
| Deamidation of Asn and Gln |
0.98402 |
-0.9847 |
| Methylation |
14.01565 |
14.0269 |
| Hydroxylation |
15.99491 |
15.9994 |
| Oxidation of Met |
15.99491 |
15.9994 |
| Formylation |
27.99491 |
28.0104 |
| Acetylation |
42.01056 |
42.0373 |
| Carboxylation of Asp and Glu |
43.98983 |
44.0098 |
| Carboxyamidomethylcysteine (Cam) from Cys (iodoacetamide)
|
57.02146 |
57.0520 |
Carboxymethylcysteine (Cme) from Cys (iodoacetic
acid)
|
58.00548 |
58.0367 |
| Phosphorylation |
79.96633 |
79.9799 |
| Sulphation |
79.95682 |
80.0624 |
| Pyridylethyleysteine (PE-Cys) from Cys (4-vinylpyridine)
|
105.05785 |
105.1393 |
| Cysteinylation |
119.00410 |
119.1442 |
| Pentoses (Ara, Rib, Xyl) |
132.04226 |
132.1161 |
| Deoxyhexoses (Fuc, Rha) |
146.05791 |
146.1430 |
| Hexosamines (GalN, GlcN) |
191.06881 |
161.1577 |
| Hexoses (Fru, Gal, Glc, Man) |
162.05282 |
162.1424 |
| Lipoic acid (amide bond to lysine) |
188.03296 |
188.3147 |
| N-acetylhexosamines (GalNAc, GlcNAc) |
203.07937 |
203.1950 |
| Farnesylation |
204.18780 |
204.3556 |
| Myristoylation |
210.19836 |
210.3598 |
| Biotinylation (amide bond to lysine) |
226.07760 |
226.2994 |
| Pyridoxal phosphate (Schiff Base formed to lysine)
|
231.02966 |
231.1449 |
| Palmitoylation |
238.22966 |
238.4136 |
| Stearoylation |
266.26096 |
266.4674 |
| Geranylgeranylation |
272.25040 |
272.4741 |
| N-acetylneuraminic acid (Sialic acid, NeuAc, NANA,
SA) |
291.09542 |
291.2579 |
| Gluthathionylation |
305.06816 |
305.3117 |
| N-glycolylneuraminic acid (NeuGc) |
307.09033 |
307.2573 |
| 5'-Adenosylation |
329.05252 |
329.2091 |
| 4'-Phosphopantetheine |
339.07797 |
339.3294 |
| ADP-ribosylation (from NAD) |
541.06111 |
541.3052 |
| Methyl (Me) |
14.01565 |
14.0269 |
| Formyl (CHO) |
27.99491 |
28.0104 |
| Ehtyl (Et) |
28.03130 |
28.0538 |
| Acetyl (Ac) |
42.01056 |
42.0373 |
| t-Butyl (t-Bu) |
56.06260 |
56.1075 |
| Anisyl |
90.04695 |
90.1246 |
| Benzyl (Bzl) |
90.04695 |
90.1246 |
| Trifluoroacetyl (Tfa) |
95.98230 |
96.0087 |
| N-hydroxysuccinimide (ONSu, Osu) |
97.01638 |
97.0734 |
| T-Butyloxycarbonyl (Boc) |
100.05243 |
100.1173 |
| Benzoyl (Bz) |
104.02621 |
104.1082 |
| 4-Methylbenzyl (Meb) |
104.06260 |
104.1515 |
| Thioanisyl |
106.02411 |
106.1912 |
| Benzyloxymethyl (Bom) |
120.05751 |
120.1509 |
| 4-Nitrophenyl (Onp) |
121.01638 |
121.0954 |
| Benzyloxycarbonyl (Z) |
134.03678 |
134.1344 |
| 2-Nitrobenzoyl (NBz) |
149.01129 |
149.1058 |
| 2-Nitrophenylsulphenyl (Nps) |
152.98845 |
153.1614 |
| 4-Toluenesulphonyl (Tosyl, Tos) |
154.00885 |
154.1894 |
| Pentafluorophenyl (Pfp) |
165.98419 |
166.0501 |
| Diphenylmethyl (Dpm) |
166.07825 |
166.2224 |
| 2-Chlorobenzyloxycarbonyl (Cl-Z) |
167.99781 |
168.5792 |
| 2, 4, 5-Trichlorophenyl |
177.91438 |
179.4320 |
| 2-Bromobenzyloxycarbonyl (Br-Z) |
211.94729 |
213.0305 |
| 9-Fluorenylmethyloxycarbonyl (Fmoc) |
222.06808 |
222.2432 |
| Triphenylmethyl (Triyl, Trt) |
242.10955 |
242.3202 |
| 2, 2, 5, 7, 8-pentamehylchroman-6-sulphonyl (Pmc) |
266.09767 |
266.3611 |
