NCBI Database - Check NCBI for the latest RefSeq numbers associated with your target gene.
Locate shRNA sets for your specific gene of interest through the MISSION shRNA Target Search database. The database may be searched using RefSeq or Accession Numbers, TRC numbers, gene symbols, gene descriptions among other specific identifiers. Target sets found in the collection based on your search criteria will be returned on a Search Results Page and listed by RefSeq number. The results can be further refined and sorted or you may click on a target to obtain in depth information about the shRNA set. Please refer to the help files in the event you have trouble using the database or locating your target.
The library is currently provided as frozen bacterial glycerol stocks harboring sequence-verified shRNA lentiviral plasmid vectors or as purified plasmid DNA. Each gene is represented by a target set that consists of an average of 3-5 individual constructs or clones targeting different regions of the gene sequence. Bacterial cultures may be amplified from the glycerol stocks for use in purification of the shRNA plasmid DNA. Alternatively, the purified plasmid format does not require these extra steps as the growth and DNA purification are completed for you. Target cell lines may be transfected with the purified plasmid for transient or stable gene silencing (puromycin selection). In addition, self-inactivating replication incompetent lentiviral particles can be produced in packaging cells (HEK 293T) by co-transfection with compatible packaging plasmids.
Orders of 5 or less target sets (≤ 25 constructs) are provided in individual 1.4 ml TrakMates® tubes. Each TrakMates® tube contains a unique 2 dimensional barcode on the bottom of the tube that can be read using a corresponding reader. A printed value corresponding to the The RNAi Consortium (TRC) clone number is also provided on each tube. Orders of >5 clone sets (>25 constructs) are automatically provided in a 96-well plate with a one dimensional barcode label on the plate and are provided with a CD containing plate map positions.
Custom gene family sets are available at anytime. Please check with your local sales representative or e-mail us at RNAi@sial.com for more information.
Check back periodically for additional target sets, formats, gene family sets, and updates to the web search capabilities.
MISSION shRNA Sets
MISSION shRNA Human Kinases: Glycerol Set-1 - SH0111
Clone sets of the MISSION shRNA library are ordered online through the MISSION shRNA Target Search database. To order online you will need to have a login profile for the website and request online order access in your profile. Upon completion of your profile, you will be able to add the products to your order cart and complete online transactions. Orders may be placed using Purchase Order numbers or credit cards. Pricing noted on the web is for 1-20 target sets. For pricing beyond 20 target sets, please contact your local sales representative, or e-mail us at RNAi@sial.com. Bacterial glycerol stocks and purified plasmid DNA are considered custom orders and are produced on-demand and shipped directly to end-users worldwide.
How to order target sets:
Update or create your web login profile with order access privilege (one time required step that may take 24-48 hours to process).
After logging in, search the database and add target sets to your cart.
Check out and submit your order.
Note: If you are providing clone product numbers to a purchasing agent, please let them know the URL of the ordering database: www.sigma-aldrich.com/missionsearch.
