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 Accuspin™ System Histopaque® – 1077

Hematology and Histology
 
Method: Density Gradient Centrifugation on Sodium DiatrizoatePolysucrose Gradients


Principle: Accuspin™ System Histopaque® – 1077 employs centrifuge tubes specially designed with two chambers separated by a porous high-density polyethlyene barrier ("frit"). The lower chamber contains Histopaque® – 1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, erythrocytes & granulocytes descend through the frit to pellet below the Histopaque® – 1077. The erythrocytes aggregate, increasing their sedimentation rate, resulting in pelleting at the bottom of the Accuspin™ tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain above the frit at the plasma – Histopaque® – 1077 interface.
Ordering Information
Accuspin System-Histopaque-1077
Product Description Product #  Size/Quantity
Polypropylene radiation sterilized tube fitted with a high density polyethylene barrier and sterile filled with HISTOPAQUE®-1077. Packaged in a 12 mL centrifuge tube. Tube contains 3 ml HISTOPAQUE®-1077-1 A 692940 x 3 mL
Polypropylene radiation sterilized tube fitted with a high density polyethylene barrier and sterile filled with HISTOPAQUE®-1077. Packaged in a 50 mL centrifuge tube. Tube contains 15 ml HISTOPAQUE®-1077-1 A 705412 x 15 mL
Polypropylene radiation sterilized tube fitted with a high density polyethylene barrier and sterile filled with HISTOPAQUE®-1077. Packaged in a 50 mL centrifuge tube. Tube contains 15 ml HISTOPAQUE®-1077-1 A 0561100 x 15 mL
Accuspinä Tubes Nonsterile, 50 mL Capacity A 193010 each
Accuspinä Tubes Sterile, 12 mL Capacity A 180520 each
Accuspinä Tubes Sterile, 50 mL Capacity A 205510 each
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