Sigma-Genosys offers a complete line of custom oligonucleotides for your research needs. Our proprietary platforms have the capacity to produce over 10,000 oligos per day in the U.S. and 5,000 per day at each of our international facilities.
Synthesis Scales
Our synthesis scales include 0.03 (3 O.D. Select), 0.05, 0.2, and 1.0 µmole. Large scale synthesis is available upon request.
Purification
All Sigma-Genosys oligos include reverse-phase cartridge (RP1)at no additional charge. For stringent applications, additional purification is available. Purification options include desalting and deprotection (no charge), reverse-phase HPLC, and PAGE. If you are unsure about the purification level you need for your research, see our technical bulletin entitled Selecting the Best Purification or contact our Technical Support staff for assistance.
Minimum Yield
Yield will vary based upon base composition, purification, oligo length, scale and modifications. That's why we have set minimum yield standards - guaranteed quantity. However, it is typical that you may receive more than the minimum yield guarantee.
Delivery
Standard oligos will ship within 36 - 48 hours. Most modified oligos will ship within 5 days. See our delivery chart for a complete list of expected shipment times. Our Customer Service department is available to answer any questions on your order status. And now - you can use our online order tracking to see first-hand where your oligo is within our process. (Web orders only)
Guaranteed Quality
Quality is not just a goal, it is a guarantee. Oligos are quality checked by PAGE and Maldi-TOF Mass Spectral analysis. If you are not satisfied with the performance of your oligo, we will remake it free of charge.
You will receive a Quality Assurance Certificate that includes quantitated yield, melting temperature, MW and µg/OD with every oligo. You will also receive an additional label that can be used in your records or on an additional vial. All this at no extra charge!
That's Not All
We have what the others don't - exceptional customer service and technical support. No other company gives you the hands-on care that Sigma-Genosys provides. From special price quotes to order confirmation to delivery updates, we will do what it takes to delight you with our service. Orders may be accepted by fax, E-mail or web.
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Modified Oligos
Sigma-Genosys offers dual-labeled probes for use in applications such as real-time PCR, multiplexing, SNP analysis, allelic discrimination and spectral genotyping. We are a licensed supplier of Molecular Beacons and Black Hole Quenchers.
Capabilities
Dual-labeled fluorogenic probes and Molecular Beacons incorporate a 5'-reporter dye and a 3'-quencher within the probe design. Sigma-Genosys can provide the traditional TAMRA quencher for dual-labeled fluorogenic probes and DABCYL quencher for Molecular Beacons.
However, a new family of quenchers is now available that has been optimized for FRET - the Black Hole QuencherTM. Sigma-Genosys is licensed to sell BHQTM labeled oligos for optimum quenching efficiency. Each BHQ has been designed to work with specific fluorophores. BHQ is suitable for both dual-labeled fluorogenic probes and Molecular Beacons.
| Quencher |
Max Abs |
Quenching Range |
Fluorophores |
| BHQ-1TM |
534 nm |
480 - 580 nm |
FAM, HEX, TET, JOE |
| BHQ-2TM |
579 nm |
550 - 650 nm |
TAMRA, ROX, Cy3TM |
Synthesis Scales
Dual-labeled fluorogenic probes and Molecular Beacons are available at the 0.05, 0.2 and 1.0 µmole scale. Please call for large scale projects.
Purification
In order to ensure that there is no background fluorescence, a two-step reverse-phase HPLC purification is standard with all dual-labeled probes.
Typical Yield
| Scale |
OD |
| 0.025 µmole |
0.5-1.0 |
| 0.05 µmole |
1.0-3.0 |
| 0.2 µmole |
3.0-5.0 |
| 1.0 µmole |
5.0-10.0 |
Yields will vary based upon oligo length and base composition. Please contact us if you have specific yield requirements.
Quality
All dual-labeled probes are quality checked by PAGE and spectrophotometer analysis. In addition, the signal-to-background ratio is measured against a target sequence for all Molecular Beacons.
You will receive a Quality Assurance Certificate that includes quantitated yield, melting temperature, MW and µg/OD with every oligo. You will also receive an additional label for use in your records or on an additional vial.
Delivery
Expected shipment time for dual-labeled probes is 5-7 days.
Pricing
Pricing for dual-labeled fluorogenic probes and Molecular Beacons includes the 5'-reporter dye, 3'-quencher, and dual RP-HPLC. Pricing includes up to 35 bases for dual-labeled fluorogenic probes (3' TAMRA) and up to 40 bases for Molecular Beacons (3' Dabcyl).
Contact us
to request a large volume quote.
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Dual Label Probes
Capabilities
Sigma-Genosys can attach fluorescent dyes to oligos at the 5'-end, 3'-end and even internal positions. Our product offering includes common dye modifiers such as 6-FAM, HEX, and TET, as well as exotic modifications. We are a licensed supplier of dyes from Molecular Probes, Inc., LI-COR, Biosearch Technologies, and Mosaic Technologies.
In addition to standard fluorescent modifications, we have the capabilities to provide dual-labeled probes including Molecular Beacons and Black Hole Quenchers.
Maximal Absorbance and Emission for Common Fluorophores
Description Max Absorbance (nm) Max Emission (nm) Recommended Purification
- Fluorescein 492 520 Desalt, RP1, HPLC
- 6-FAM 494 525 Desalt, RP1, HPLC
- TET 521 536 Desalt, RP1, HPLC
- HEX 535 556 Desalt, RP1, HPLC
- TAMRA 555 580 HPLC
- Texas Red 595 615 HPLC
- JOE 521 547 Desalt, RP1, HPLC
- Cy5TM 643 667 RP1, HPLC
- Cy3TM 552 570 RP1, HPLC
- BODIPY FL 505 513 HPLC
- Oregon Green 488 493 520 HPLC
Synthesis Scales
Most modifications are available at the 0.05, 0.2, and 1.0 µmole scale. Please call for large scale projects.
Purification
Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite modifiers. However, additional purification such as HPLC is strongly recommended for modifiers that require NHS-ester chemistry to conjugate a dye through an amine linker such as Molecular Probes dyes.
Minimum Yield
Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our modification yield chart for minimum yield guarantees.
Quality
All modified oligos are quality checked by PAGE and spectrophotometer analysis. In addition, we perform specialized QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.
You will receive a Quality Assurance Certificate that includes quantitated yield, melting temperature, MW and µg/OD with every oligo. You will also receive an additional label for use in your records or on an additional vial.
Delivery
Expected shipment time for both common dye modifiers and more exotic modifications is usually 5 - 7 working days.
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