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Biostabilization Products
During long-term storage many biomolecules are subject to activity loss. The BioStab Products are used to preserve the activity of biomolecules like enzymes, antibodies, DNA, whole cells or cell membranes, during long-term storage in solution.
| Catalog No. | Product | Application | | | BioStab Biomolecule Storage Solution | During a storage over a long time diverse biomolecules are subject to losing their activity. The biomolecule storage solution is used to preserve the activity of biomolecules like enzymes, antibodies, DNA, whole cells or cell membranes during the storage over a long time.
(Application protocol) | | | BioStab Enzyme Stabilizer | A thermostable, chemical inert substance was isolated and purified from extremophile microorganisms. The enzyme stabilizer is used, analogue to the natural function, to protect biomolecules like enzymes from denaturation by thermal stress, proteolysis, pH changes or the salt concentration.
(Application protocol) | | | BioStab General Proteolysis Inhibitor | The enzymatic hydrolysis of proteins can be prevented by BioStab General Proteolysis Inhibitor. An unspecific stabilization of the substrate is responsible for the inhibition. Various experiments demonstrate an excellent activity of the functional protein after addition of the inhibitor.
(Application protocol) | | | BioStab Immunoassay Stabilizer | Immunoassays, like an ELISA-test, can be protected from external degradation using BioStab Immunoassay Stabilizer. The addition of BioStab Immunoassay Stabilizer helps to increase the ability of stocking and the coating rates of the antibodies.
(Application protocol) | | | BioStab PCR Optimizer | A thermostable, chemical inert substance was isolated and purified from extremophile micro-organisms. The PCR optimizer stabilizes biomolecules like enzymes and is used to increase the thermostability and the half-life period of the Taq Polymerase, so it is possible to realize more cycles.
(Application protocol) |
BioStab products are easy to use. This may be demonstrated for the stabilization of prokaryotic cells:
Instructions:
Step 1) Take the suspension of a vital cell culture and add 1% NaCl
Step 2) Add 7 parts of the prepared suspension to 1 part of BioStab
Biomolecule Storage Solution.
Step 3) Store the cells according to your common procedure.
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92889 BioStab Biomolecule Storage Solution During long-term storage many biomolecules are subject to losing their activity. The biomolecule storage solution is used to preserve the activity of biomolecules like enzymes, antibodies, DNA, whole cells or cell membranes during long-term storage.
Product Description: BioStab Biomolecule Storage Solution is a 4x solution in sterile bidest. water (free of DNAse, RNAse and protease). 2x5ml is sufficient for making 40 vials à 1ml, 10x5ml is sufficient for 200 vials à 1ml. Store at 4°C.
Directions: Example of a preparation for the storage of CD30-binding proteins over a long time:
| No. | Substance | Volume [ml] | | 1 | PBS-Buffer (pH 7.0) | 745 | | 2 | CD30-binding proteins (1mg/ml) | 5 | | 3 | BioStab Biomolecule Storage Solution | 250 |
Stabilization of prokaryotic cells - Take the suspension of a vital cell culture and add 1% NaCl
- Add 7 parts of the prepared suspension to 1 part of BioStab Biomolecule Storage Solution.
- Store the cells according to your common procedure.
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95576 BioStab Enzyme Stabilizer A thermostable, chemical inert substance isolated and purified from extremophile microorganisms. The enzyme stabilizer is used, analogue to the natural function, to protect biomolecules like enzymes from denaturation by thermal stress, proteolyses, pH changes or salt concentration.
Product Description: BioStab Enzyme Stabilizer is a 4x solution in sterile bidest. water (free of DNAse, RNAse and protease). A 10ml package is sufficient for making 40ml protein solution, 50ml is sufficient for 200ml protein solution. Store at 4°C.
Directions: Example of a preparation for the storage of enzyme over a long time:
| No. | Substance | Volume [ml] | | 1 | Buffer solution (the buffer which is used for the application) | 745 | | 2 | Enzyme | 5 | | 3 | Biostab Enzyme Stabilizer | 250 |
BioStab Enzyme Stabilizer can be used during an enzyme assay, the purification and the storage of enzymes.
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75998 BioStab General Proteolysis Inhibitor The enzymatic hydrolysis of proteins can be prevented by BioStab General Proteolysis Inhibitor. An unspecific stabilization of the substrate is responsible for the inhibition. Various experiments demonstrate an excellent activity of the functional protein after addition of the inhibitor.
Product Description: BioStab General Proteolysis Inhibitor is a 4x solution in sterile bidest. water (free of DNAse, RNAse and protease). A 50ml package is sufficient for making 200ml protein solution, 250ml is sufficient for 1000ml protein solution. Store at 4°C.
Directions: Example of a reaction-mix for a proteolysis-inhibition-test:
| No. | Substance | Volume [ml] | | 1 | Human IgG (0.5 mg/ml) | 745 | | 2 | Pepsin (5 mg/ml) | 5 | | 3 | BioStab General Proteolysis Inhibitor | 250 |
After 15 min. incubation at 37°C the antibody still showed an activity of 90%.
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93859 BioStab Immunoassay Stabilizer Immunoassays, e.g. an ELISA-tests, can be protected from degradation effects using BioStab Immunoassay Stabilizer. The addition of BioStab Immunoassay Stabilizer increases life-time and the coating rates of the antibodies.
BioStab Immunoassay Stabilizer is a 4x solution in sterile bidest. water (free of DNAse, RNAse and protease). A 50ml package is enough for making about 2000 tests, 250ml is sufficient for about 10000 tests. Store at 4°C.
Directions: An example for the stabilization of adsorbed or immobilized proteins on microwell-plates. - Immobilize the primary protein (antibody or antigen) according to your method. The surplus or insufficient coated protein is removed by a washing step.
- Immediately after washing, add sufficient BioStab Immunoassay Stabilizer to get an interaction between the protein surface and the stabilizer. In case you used 75ml/well from the primary solution (in the first step) you have to add 25ml/well of the BioStab Immunoassay Stabilizer. Coated components should never dry up before adding the stabilizer.
- Incubate for 15 to 60 min. at room temperature. Block with the corresponding solution. It is recommended to mix 3 parts of the block solution with 1 part of the BioStab Immunoassay Stabilizer.
- Remove the supernatant but do not wash the plate.
- Now the plate can be stored over a long time according to your standard method
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92832 BioStab PCR Optimizer A thermostable, chemical inert substance was isolated and purified from extremophile microorganisms. The PCR optimizer stabilizes biomolecules like enzymes and is used to increase the thermostability and the half-life period of Taq Polymerase to realize more cycles.
Product Description: BioStab PCR Optimizer is a 4x solution in sterile bidest. water (free of DNAse, RNAse and protease). A 5ml package is enough for making 400 reactions, 20ml is sufficient for 1600 reactions. Store at 4°C.
Directions: For a reaction mix as recommended:
| No. | Substance | Volume [ml] | | 1 | Sterile bidest. water | 21.5 | | 2 | 10x reaction buffer | 5 | | 3 | 50 mM MgCl2 | 2 | | 4 | 4x BioStab PCR Optimizer | 12.5 | | 5 | dNTP-Mix (2 mM each) | 5 | | 6 | Forward primer (10 pmol) | 1 | | 7 | Reverse primer (10 pmol) | 1 | | 8 | Template DNA (50-200 ng) | 1 | | 9 | Taq DNA-Polymerase (2.5 U) | 1 |
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