Crystallization
Crystallization Screening Kits
Crystallization Optimization (Stock) Solutions
Crystallization Screening Kits Introduction These kits provide a rapid and cost efficient method for optimization of crystallization conditions for biological macromolecules.
Sigma crystallization screening kits contain ready to use solutions based on published conditions and prooven suitable for this purpose.
Features: - Ready made solutions based on known or published successful crystallization conditions of various proteins
- Prepared from high purity reagents (mostly Fluka Microselect grade) and ultrapure water
- Convenient kit packaging
- Individual solutions and individual reagents available
Features: - Rapid and cost efficient optimization of crystallization procedures
- Reliable quality, extensive specification of all reagents used for preparation, quality grade proved sufficient for crystallization purposes since years
- Concise lay-out, stand-alone vials, vials can easily be taken out and put back again.
- Up-scaling of crystallization following identification of good conditions is easy to obtain
The following screening kits are available:
- Crystallization Basic Kit for Proteins: 82009
A rapid empirical screening method to determine the best conditions for the crystallization of biological macromolecules like proteins. Given conditions are based upon the original screening protocol of Jancarik and Kim. Click here for obtaining information about the Technical Bulletin of 82009. - Crystallization Extension Kit for Proteins: 70437
An easy extension trial to find crystallization conditions for macromolecules. Click here for obtaining information about the Technical Bulletin of 70437. - Crystallization Cryo Kit for Proteins: 75403
An easy modified trial to find crystallization conditions. The difference to the basic kit is the presence of glycerol as a cryoprotectant. Click here for obtaining information about the Technical Bulletin of 75403. - Crystallization Low Ionic Kit for Proteins: 86684
An easy tool for determination of crystallization conditions for antibodies and other soluble proteins. Conditions are based upon a screening protocol from Lisa Harris et al. Click here for obtaining information about the Technical Bulletin of 86684. - Crystallization Basic Kit for Membrane Proteins: 73513
A rapid empirical screening method to determine the best conditions for the crystallization of membrane proteins. Click here for obtaining information about the Technical Bulletin of 73513. - Crystallization Kit for Automatic Screening: 56783
Increasing throughput and demand for fast, reliable and reproducible handling have led us to a new format of crystallization screening kit. The Crystallization screening kit for proteins has been filled into a 96-deepwell plate.
This kit contains nearly all the components from our basic crystallography kit (Cat. No. 82009) and extension Kit (Cat. No. 70437). The kit includes 1 ml of each reagent. The 8 x 12 format allows an easy and fast transfer of solutions to the screening plates that are used for your experiment. A 8-tips-pipette may be used for manual handling. This will save you most of the pipetting time. The format also fits any automatic liquid handling station you might have available.
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Crystallization Optimization (Stock) Solutions For further optimization of crystallization conditions we are introducing a wide range of new single component stock solutions capable for easy preparation of your individual optimization screening conditions. We already offer a range of such solutions as listed below. These solutions are made up from our ultra pure reagents (BioChemika Ultra; see also BioChemika Ultra: Crystallization), and pure water. They are sterilized and batch-to-batch consistency is subject of extensive analysis.
Product List | Catalog No. | Product Name | Pack Size | | 88571 | 1,6-Hexanediol solution 6M | 100 ml | | 88163 | Ammonium chloride solution 5M | 250 ml, 1 l | | 70705 | Ammonium phosphate dibasic solution 2.5M | 100 ml, 500 ml | | 83267 | Ammonium sulfate solution 3.2M | 100 ml, 500 ml | | 83263 | BICINE solution 1M | 100 ml, 500 ml | | 70216 | HEPES sodium salt buffer solution 1M | 100 ml, 500 ml | | 83264 | HEPES solution 1M | 100 ml, 500 ml | | 68268 | Imidazol solution 1M | 100 ml, 500 ml | | 83268 | Lithium chloride solution 10M | 100 ml, 500 ml | | 82348 | Lithium sulfate solution 2M | 100 ml, 500 ml | | 68475 | Magnesium chloride solution 2M | 50 ml, 100 ml | | 83270 | Magnesium nitrate 3M | 100 ml, 250 ml | | 83266 | Magnesium sulfate solution 2.5M | 100 ml, 500 ml | | 76039 | MES buffer solution 0.5M | 100 ml, 500 ml | | 76293 | Polyethylene glycol 1000 solution 50% | 100 ml, 250 ml | | 83271 | Polyethylene glycol 10000 solution 50% | 100 ml, 250 ml | | 73034 | Polyethylene glycol 1500 solution 50% | 100 ml, 250 ml | | 83918 | Polyethylene glycol 2000 solution 50% | 100 ml, 500 ml | | 87006 | Polyethylene glycol 20000 solution 50% | 100 ml, 250 ml | | 83272 | Polyethylene glycol 3350 solution 50% | 100 ml, 500 ml | | 89782 | Polyethylene glycol 4000 solution 50% | 100 ml, 500 ml | | 90364 | Polyethylene glycol 5000 monomethyl ether solution 50% | 100 ml, 250 ml | | 83271 | Polyethylene glycol 8000 solution 50% | 100 ml, 500 ml | | 95843 | Potassium acetate solution 5M | 100 ml, 250 ml | | 89306 | Potassium citrate tribasic solution 2.5M | 100 ml, 500 ml | | 78716 | Potassium formiate 14M | 100 ml, 500 ml | | 81028 | Potassium sodium tartrate solution 1.5% | 100 ml, 500 ml | | 73059 | Potassium thiocyanate solution 8M | 100 ml, 500 ml | | 74092 | Sodium phosphate monobasic solution 5M | 100 ml, 500 ml | | 83269 | Sodium sulfate solution 1M | 250 ml, 1 l | | 80518 | Sodium thiocyanate solution 8M | n.d. | | 83273 | Sodium citrate tribasic solution 2.5M | 100 ml, 1 l | | 70331 | Zinc acetate solution 1M | 100 ml | | 83265 | Zinc sulfate solution 2M | 250 ml, 1 l |
We will continue to add more solutions for optimization of crystallization. Based on our wider range of ultra pure reagents for crystallization and extensive experience for production of formulated reagents, we are also ready to prepare customized reagents.
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