CUSTOM ANTISERA

Sigma-Genosys offers a comprehensive service for polyclonal antibody production. The service includes:

• Use of design software to optimize the synthesis yield and antigenicity of the peptide
• Consultation on conjugation chemistry to the carrier protein for proper presentation to the immune system
• Dedicated professionals to communicate and coordinate your project specifications
• Guaranteed immune response of 1:10,000 to the peptide antigen that is designed by Sigma

Sigma-Genosys offers several options for custom antibody production

• The Complete Package
• The Monospecific/Characterized Package
• The Phosphospecific Package
• The Partial Package

Critical Aspects of Antigen Design

Antigen Design and Synthesis

• Use of sophisticated software to analyze protein sequence for antigenicity, flexibility and B-turn formation
• Peptide chemistry analysis is performed on the selected peptide segment to ensure synthetic manufacturing success
• Peptide is synthesized using proprietary Pepticus synthesis technology with Fmoc chemistry
• Peptide is analyzed by mass spectrometry and reversed phase HPLC to confirm composition and purity respectively

• See Conjugation Guidelines page for a complete explanation of peptide conjugation

• Two New Zealand white rabbits are standard host animals
• Chicken, Sheep and Goats are available
• Avoid self-recognition of the immunogen by the host animal by selecting a host species that is very different from your immunogen source.
• See Animal Options for more details

• Antigen is injected subcutaneously into two host animals
• Initial immunization is given in Complete Freund's Adjuvant and all subsequent immunizations in Incomplete Freund's Adjuvant.
• Pre-immune bleed is drawn and supplied to customer
• 77-day schedule includes 6 immunizations per animal and 4 bleeds per animal
• ELISA is performed on pre-immune and first production bleed
• Sera are supplied unpurified (purification available upon request) and contain several different isotypes (IgG, IgM and IgA)
• Detailed immunization/bleed schedule

• Navigate through more than 3,000 antibodies
• Search by specificity or clone
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