|
The Complete Package
Peptide Synthesis (IG purity, 10mg, <=15 amino acids)
Conjugation to KLH or BSA (2-3mg)
2 Animals (Rabbit or Chicken*)
6 Immunizations/animal
4 Bleeds/animal (including pre-immune bleed)
*Yolks are provided for chicken projects
ELISA of Bleed #1
The Monospecific/Characterized Package
Peptide Synthesis (IG purity, 10mg, <=15 amino acids)
Conjugation to KLH or BSA (2-3mg)
2 Animals (Rabbit or Chicken*)
6 Immunizations/animal
4 Bleeds/animal (including pre-immune bleed)
*Yolks are provided for chicken projects
ELISA of Bleed #1
Exsanguination of both animals
Affinity Purification of Bleed #3
— Typical yield of 25ml of sera is 2-3mg of purified antibody
Western Blot testing of purified antibody
— Full panel of 15 lysates
— Electronic image of the blot is provided
The Phosphospecific Package
Peptide Synthesis
Phosphopeptide: >95% purity, 10mg, <=15 amino acids
Non-phosphopeptide: >70% purity, 10mg, <=15 amino acids
Conjugation of phosphopeptide to KLH or BSA (2-3mg)
2 Animals (Rabbit or Chicken*)
6 Immunizations/animal
4 Bleeds/animal (including pre-immune bleed)
*Yolks are provided for chicken projects
ELISA of Bleed #1 using phosphopeptide and non-phosphopeptide
Affinity Purification of selected batch of serum
Depletion of non-phosphospecific antibody
Purification of phosphospecific antibody
The Partial Package
Customer supplied antigen
2 Animals (Rabbit or Chicken*)
6 Immunizations/animal
4 Bleeds/animal (including pre-immune bleed)
*Yolks are provided for chicken projects
ELISA of Bleed #1
Standard Protocol:
Day 0 Pre-bleed, Antigen Injection/200µg peptide (CFA)
Day 14 Antigen Injection/100µg peptide (IFA)
Day 28 Antigen Injection/100µg peptide (IFA)
Day 42 Antigen Injection/100µg peptide (IFA)
Day 49 Bleed #1/ELISA
Day 56 Antigen Injection/100µg peptide (IFA)
Day 63 Bleed #2
Day 70 Antigen Injection/100µg peptide (IFA)
Day 77 Bleed #3*
*Daily housing charges ($3/day/rabbit or chicken) begin accruing 1week after shipping the 3rd production bleed, until termination instructions are given by the customer.
|
