Attention: The following specifications apply to the USA and Europe. All other countries, click here. |
|
| |
For use on Beckman Coulter's CEQ* Genetic Analysis
Systems.
- High-quality oligos
- Reliable service
- Competitively priced
|
|
Sigma-Genosys is licensed by Beckman Coulter, Inc., to supply WellRED dye-labeled oligos designed for use with the CEQ* Genetic Analysis Systems and may be used for
direct hybridization or in PCR amplification. WellRED dye-labeled oligos are DNA
oligonucleotides labeled with WellRED dyes, D2, D3 or D4, at the 5'end. WellRed dye-labeled
oligo specifications:
- Deprotected and desalted
- Purified by PAGE, RPC or RP-HPLC
- Available in lengths of 7 to 100 mers
- Delivered dried in individual, opaque tubes
- Shipped within 4 to 5 working days of receiving your order, pending successful
QC validation
- Systematic QC by PAGE or mass spectrometry analysis
- Competitively priced
Benefits of WellRED Dye Chemistry Used with the CEQ* Genetic Analysis
Systems
1. WellRED oligos use cyanine-based fluorescent dyes with high
extinction coefficients that absorb in the near-infrared region, thereby reducing
background noise from biological materials, resulting in highly sensitive detection.
2. The CEQ* Genetic Analysis Systems enable DNA samples to be kept in
linear form during analysis. The CEQ* Genetic Analysis Systems' online denaturation process
prevents any reformation of secondary structures, especially during a long run without any
manual additional step.
3. WellRED dye chemistry and pre-heating enable the CEQ* Genetic
Analysis System to resolve complex structures, where other systems fail.
Guaranteed Yields and Estimated Assays for WellRED
Dye-labeled Oligos
|
PAGE |
RPC |
RP-HPLC |
| Guaranteed yield
(OD) |
1 |
2 |
4 |
5 |
10 |
50 |
100 |
| Approx. yield
(nmols*) |
5 |
10 |
20 |
25 |
50 |
250 |
500 |
| Estimated assays
(reactions) |
500
-
1,000 |
1,000
-
2,000 |
2,000
-
4,000 |
2,500
-
5,000 |
5,000
-
10,000 |
25,000
-
50,000 |
50,000
-
100,000 |
Spectral Properties of WellRED Dyes
| WellRED oligo |
Exitation maximum (nm) |
emission maximum (nm) |
Molar extinction coefficient |
| D2-PA |
750 |
770 |
170,000 |
| D3-PA |
685 |
706 |
224,000 |
| D4-PA |
650 |
670 |
203,000 |
*CEQ is a trademark of Beckman Coulter, Inc.
|
